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Applied and Environmental Microbiology, January 2000, p. 92-97, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A New Chemolithoautotrophic Arsenite-Oxidizing Bacterium Isolated from a Gold Mine: Phylogenetic, Physiological, and Preliminary Biochemical Studies

Joanne M. Santini,1 Lindsay I. Sly,2 Roger D. Schnagl,1 and Joan M. Macy1,*

Department of Microbiology, La Trobe University, Bundoora, Victoria 3083,1 and Centre for Bacterial Diversity and Identification, Department of Microbiology and Parasitology, The University of Queensland, Brisbane, Queensland 4072,2 Australia

Received 21 June 1999/Accepted 20 October 1999

A previously unknown chemolithoautotrophic arsenite-oxidizing bacterium has been isolated from a gold mine in the Northern Territory of Australia. The organism, designated NT-26, was found to be a gram-negative motile rod with two subterminal flagella. In a minimal medium containing only arsenite as the electron donor (5 mM), oxygen as the electron acceptor, and carbon dioxide-bicarbonate as the carbon source, the doubling time for chemolithoautotrophic growth was 7.6 h. Arsenite oxidation was found to be catalyzed by a periplasmic arsenite oxidase (optimum pH, 5.5). Based upon 16S rDNA phylogenetic sequence analysis, NT-26 belongs to the Agrobacterium/Rhizobium branch of the alpha -Proteobacteria and may represent a new species. This recently discovered organism is the most rapidly growing chemolithoautotrophic arsenite oxidizer known.

* Corresponding author. Mailing address: Department of Microbiology, La Trobe University, Bundoora, Victoria 3083, Australia. Phone: 61 3 9479 2229. Fax: +61 3 9479 1222. E-mail: j.macy{at}latrobe.edu.au.

Applied and Environmental Microbiology, January 2000, p. 92-97, Vol. 66, No. 1
0099-2240/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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