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Applied and Environmental Microbiology, October 2000, p. 4503-4509, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection and Identification of Bacterial Endosymbionts in Arbuscular Mycorrhizal Fungi Belonging to the Family Gigasporaceae

Valeria Bianciotto,1 Erica Lumini,2 Luisa Lanfranco,2 Daniela Minerdi,2 Paola Bonfante,1,2,* and Silvia Perotto1

Centro Studio Micologia del Terreno-CNR1 and Dipartimento di Biologia Vegetale dell'Università,2 10125 Turin, Italy

Received 5 April 2000/Accepted 17 July 2000

Intracellular bacteria have been found previously in one isolate of the arbuscular mycorrhizal (AM) fungus Gigaspora margarita BEG 34. In this study, we extended our investigation to 11 fungal isolates obtained from different geographic areas and belonging to six different species of the family Gigasporaceae. With the exception of Gigaspora rosea, isolates of all of the AM species harbored bacteria, and their DNA could be PCR amplified with universal bacterial primers. Primers specific for the endosymbiotic bacteria of BEG 34 could also amplify spore DNA from four species. These specific primers were successfully used as probes for in situ hybridization of endobacteria in G. margarita spores. Neighbor-joining analysis of the 16S ribosomal DNA sequences obtained from isolates of Scutellospora persica, Scutellospora castanea, and G. margarita revealed a single, strongly supported branch nested in the genus Burkholderia.

* Corresponding author. Mailing address: Dipartimento Biologia Vegetale, Università di Torino, V. le Mattioli 25, 10125 Turin, Italy. Phone: (39) 011 6502927. Fax: (39) 011 6707459. E-mail: p.bonfante{at}csmt.to.cnr.it.

Applied and Environmental Microbiology, October 2000, p. 4503-4509, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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