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Applied and Environmental Microbiology, October 2000, p. 4510-4513, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Fruiting Body Tissue Method for Efficient Agrobacterium-Mediated Transformation of Agaricus bisporus

Xi Chen, Michelle Stone, Carl Schlagnhaufer, and C. Peter Romaine*

Department of Plant Pathology, The Pennsylvania State University, University Park, Pennsylvania 16802

Received 28 April 2000/Accepted 1 August 2000

We describe a modified Agrobacterium-mediated method for the efficient transformation of Agaricus bisporus. Salient features of this procedure include cocultivation of Agrobacterium and fruiting body gill tissue and use of a vector with a homologous promoter. This method offers new prospects for the genetic manipulation of this commercially important mushroom species.

* Corresponding author. Mailing address: Department of Plant Pathology, 209 Buckhout Laboratory, The Pennsylvania State University, University Park, PA 16802. Phone: (814) 865-7132. Fax: (814) 863-7217. E-mail: cpr2{at}psu.edu.

Applied and Environmental Microbiology, October 2000, p. 4510-4513, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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  • Heneghan, M. N., Porta, C., Zhang, C., Burton, K. S., Challen, M. P., Bailey, A. M., Foster, G. D. (2009). Characterization of Serine Proteinase Expression in Agaricus bisporus and Coprinopsis cinerea by Using Green Fluorescent Protein and the A. bisporus SPR1 Promoter. Appl. Environ. Microbiol. 75: 792-801 [Abstract] [Full Text]  
  • Tzfira, T., Citovsky, V. (2003). The Agrobacterium-Plant Cell Interaction. Taking Biology Lessons from a Bug. Plant Physiol. 133: 943-947 [Full Text]  


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