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Applied and Environmental Microbiology, October 2000, p. 4518-4522, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of Combined Microautoradiography and Fluorescence In Situ Hybridization To Determine Carbon Metabolism in Mixed Natural Communities of Uncultured Bacteria from the Genus Achromatium

N. D. Gray,1 R. Howarth,1 R. W. Pickup,2 J. Gwyn Jones,3 and I. M. Head1,*

Fossil Fuels and Environmental Geochemistry Postgraduate Institute and Centre for Molecular Ecology, University of Newcastle, Newcastle upon Tyne NE1 7RU,1 and Institute of Freshwater Ecology2 and Freshwater Biological Association,3 Windermere Laboratories, Far Sawrey, Ambleside, Cumbria LA22 0LP, United Kingdom

Received 21 March 2000/Accepted 18 July 2000

Combined microautoradiography and fluorescence in situ hybridization (FISH) was used to investigate carbon metabolism in uncultured bacteria from the genus Achromatium. All of the Achromatium species identified in a freshwater sediment from Rydal Water, Cumbria, United Kingdom, which were distinguishable only by FISH, assimilated both [14C]bicarbonate and [14C]acetate. This extends previous findings that Achromatium spp. present at another location could only utilize organic carbon sources. Achromatium spp., therefore, probably exhibit a range of physiologies, i.e., facultative chemolithoautotrophy, mixotrophy, and chemoorganoheterotrophy, similar to other large sulfur bacteria (e.g., Beggiatoa spp.).

* Corresponding author. Mailing address: Fossil Fuels and Environmental Geochemistry Postgraduate Institute (NRG), University of Newcastle, Newcastle upon Tyne NE1 7RU, United Kingdom. Phone: 44 (0) 191-222-7024. Fax: 44 (0) 191-222-5431. E-mail: i.m.head{at}newcastle.ac.uk.

Applied and Environmental Microbiology, October 2000, p. 4518-4522, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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