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Applied and Environmental Microbiology, October 2000, p. 4543-4546, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Microscale Diversity of the Genus Nitrobacter in Soil on the Basis of Analysis of Genes Encoding rRNA

G. L. Grundmann* and P. Normand

Laboratoire d'Ecologie Microbienne, UMR 5557 Université Claude Bernard, 69622 Villeurbanne Cedex, France

Received 20 March 2000/Accepted 17 July 2000

We looked at the diversity of NO2- oxidizers at field scale by examining isolates at clump scale and in microsamples of soil (diameter, 50 µm). The genetic distances (as determined by amplified ribosomal DNA restriction analysis performed with Nitrobacter-specific primers) in a small clump of soil were as large as those between reference strains from large geographical areas. Diversity in individual microsamples was shown by serotyping.

* Corresponding author. Mailing address: Laboratoire d'Ecologie Microbienne, UMR 5557, Université Claude Bernard, 43 Bd du 11 Novembre 1918, 69622 Villeurbanne Cedex, France. Phone: (33) 04 72 43 13 78. Fax: (33) 04 72 43 12 23. E-mail: grundman{at}biomserv.univ-lyon1.fr.

Applied and Environmental Microbiology, October 2000, p. 4543-4546, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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