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Applied and Environmental Microbiology, October 2000, p. 4568-4570, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Incorporation of Protease K into Larval Insect Membrane Vesicles Does Not Result in Disruption of Integrity or Function of the Pore-Forming Bacillus thuringiensis delta -Endotoxin

Arthur Aronson*

Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907

Received 24 April 2000/Accepted 17 July 2000

Bacillus thuringiensis delta -endotoxins insert into the brush border membranes of insect larval cells to form ion channels. A possible interaction of these toxins with a cytoplasmic component was examined by preloading vesicles from insect larval cells with protease K followed by incubation with toxin. There was no evidence for toxin antigens smaller than the intact toxin in extracts of solubilized vesicles, nor was there an effect of the inclusion of protease K on either of two functional properties, the formation of toxin aggregates or of ion pores. These toxins, physically and functionally, appear to be confined to the membrane.

* Mailing address: Department of Biological Sciences, Lilly Hall, Purdue University, West Lafayette, IN 47907-1392. Phone: (765) 494-4992. Fax: (765) 494-0876. E-mail: aaronson{at}bilbo.bio.purdue.edu.

Applied and Environmental Microbiology, October 2000, p. 4568-4570, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nair, M. S., Dean, D. H. (2008). All Domains of Cry1A Toxins Insert into Insect Brush Border Membranes. J. Biol. Chem. 283: 26324-26331 [Abstract] [Full Text]  


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