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Applied and Environmental Microbiology, October 2000, p. 4579-4581, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Antisense Silencing of the creA Gene in Aspergillus nidulans

L. Fernando Bautista,dagger Alexei Aleksenko,* Morten Hentzer,Dagger Anne Santerre-Henriksen, and Jens Nielsen

Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, 2800 Lyngby, Denmark

Received 18 May 2000/Accepted 31 July 2000

Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, growth parameters and colony morphology in the antisense transformants were not affected.

* Corresponding author. Mailing address: Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, 2800 Lyngby, Denmark. Phone: (45) 4525 2700. Fax: (45) 4588 4148. E-mail: aal{at}ibt.dtu.dk.

dagger Present address: Department of Chemical Engineering, Faculty of Chemistry, Universidad Complutense de Madrid, 28040 Madrid, Spain.

Dagger Present address: Department of Microbiology, Technical University of Denmark, 2800 Lyngby, Denmark.

Applied and Environmental Microbiology, October 2000, p. 4579-4581, Vol. 66, No. 10
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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