This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Carelli, M.
Right arrow Articles by Bazzicalupo, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Carelli, M.
Right arrow Articles by Bazzicalupo, M.
Agricola
Right arrow Articles by Carelli, M.
Right arrow Articles by Bazzicalupo, M.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, November 2000, p. 4785-4789, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genetic Diversity and Dynamics of Sinorhizobium meliloti Populations Nodulating Different Alfalfa Cultivars in Italian Soils

Maria Carelli,1 Stefano Gnocchi,1 Silvia Fancelli,2 Alessio Mengoni,2 Donatella Paffetti,2 Carla Scotti,1 and Marco Bazzicalupo2,*

Istituto Sperimentale Colture Foraggere, 200075 Lodi,1 and Dipartimento di Biologia Animale e Genetica, University of Florence, 50125 Florence,2 Italy

Received 5 April 2000/Accepted 24 August 2000

We analyzed the genetic diversity of 531 Sinorhizobium meliloti strains isolated from nodules of Medicago sativa cultivars in two different Italian soils during 4 years of plant growth. The isolates were analyzed for DNA polymorphism with the random amplified polymorphic DNA method. The populations showed a high level of genetic polymorphism distributed throughout all the isolates, with 440 different haplotypes. Analysis of molecular variance allowed us to relate the genetic structure of the symbiotic population to various factors, including soil type, alfalfa cultivar, individual plants within a cultivar, and time. Some of these factors significantly affected the genetic structure of the population, and their relative influence changed with time. At the beginning of the experiment, the soil of origin and, even more, the cultivar significantly influenced the distribution of genetic variability of S. meliloti. After 3 years, the rhizobium population was altered; it showed a genetic structure based mainly on differences among plants, while the effects of soil and cultivar were not significant.

* Corresponding author. Mailing address: Dipartimento di Biologia Animale e Genetica, Via Romana 17, 50125 Florence, Italy. Phone: 39-0552288242. Fax: 39-0552288250. E-mail: marcobazzi{at}dbag.unifi.it.

Applied and Environmental Microbiology, November 2000, p. 4785-4789, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Biondi, E. G., Tatti, E., Comparini, D., Giuntini, E., Mocali, S., Giovannetti, L., Bazzicalupo, M., Mengoni, A., Viti, C. (2009). Metabolic Capacity of Sinorhizobium (Ensifer) meliloti Strains as Determined by Phenotype MicroArray Analysis. Appl. Environ. Microbiol. 75: 5396-5404 [Abstract] [Full Text]  
  • van Berkum, P., Badri, Y., Elia, P., Aouani, M. E., Eardly, B. D. (2007). Chromosomal and Symbiotic Relationships of Rhizobia Nodulating Medicago truncatula and M. laciniata. Appl. Environ. Microbiol. 73: 7597-7604 [Abstract] [Full Text]  
  • Sun, S., Guo, H., Xu, J. (2006). Multiple gene genealogical analyses reveal both common and distinct population genetic patterns among replicons in the nitrogen-fixing bacterium Sinorhizobium meliloti.. Microbiology 152: 3245-3259 [Abstract] [Full Text]  
  • van Berkum, P., Elia, P., Eardly, B. D. (2006). Multilocus sequence typing as an approach for population analysis of medicago-nodulating rhizobia.. J. Bacteriol. 188: 5570-5577 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»