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Applied and Environmental Microbiology, November 2000, p. 4849-4853, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Fate of Selenate and Selenite Metabolized by Rhodobacter sphaeroides

Verena Van Fleet-Stalder,1,dagger Thomas G. Chasteen,1,* Ingrid J. Pickering,2 Graham N. George,2 and Roger C. Prince3

Chemistry Department and Texas Research Institute for Environmental Studies, Sam Houston State University, Huntsville, Texas 773411; Stanford Synchrotron Radiation Laboratory, Stanford Linear Accelerator Center, Stanford, California 943092; and ExxonMobil Research and Engineering Company, Annandale, New Jersey 088013

Received 29 June 2000/Accepted 6 September 2000

Cultures of a purple nonsulfur bacterium, Rhodobacter sphaeroides, amended with ~1 or ~100 ppm selenate or selenite, were grown phototrophically to stationary phase. Analyses of culture headspace, separated cells, and filtered culture supernatant were carried out using gas chromatography, X-ray absorption spectroscopy, and inductively coupled plasma spectroscopy-mass spectrometry, respectively. While selenium-amended cultures showed much higher amounts of SeO32- bioconversion than did analogous selenate experiments (94% uptake for SeO32- as compared to 9.6% for SeO42--amended cultures from 100-ppm solutions), the chemical forms of selenium in the microbial cells were not very different except at exposure to high concentrations of selenite. Volatilization accounted for only a very small portion of the accumulated selenium; most was present in organic forms and the red elemental form.

* Corresponding author. Mailing address: Department of Chemistry, Sam Houston State University, P.O. Box 2117, Huntsville, TX 77341. Phone: (936) 294-1533. Fax: (936) 294-4996. E-mail: chm_tgc{at}shsu.edu.

dagger Present address: Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, St. Paul, MN 55108-1022.

Applied and Environmental Microbiology, November 2000, p. 4849-4853, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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