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Applied and Environmental Microbiology, November 2000, p. 4908-4915, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Occurrence of Antimicrobial Resistance in Fish-Pathogenic and Environmental Bacteria Associated with Four Danish Rainbow Trout Farms

Anja S. Schmidt,1,* Morten S. Bruun,1 Inger Dalsgaard,2 Karl Pedersen,3 and Jens L. Larsen1

Department of Veterinary Microbiology1 and Danish Institute of Fisheries Research,2 The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C, and Danish Veterinary Laboratory, DK-8200 Aarhus,3 Denmark

Received 23 May 2000/Accepted 25 August 2000

Surveillance of bacterial susceptibility to five antimicrobial agents was performed during a 1-year period in and around four freshwater fish farms situated along a stream in western Denmark. Besides assessing the levels of antibiotic resistance among the culturable fraction of microorganisms in fish, water, and sediment samples, two major fish pathogens (88 Flavobacterium psychrophilum isolates and 134 Yersinia ruckeri isolates) and 313 motile Aeromonas isolates, representing a group of ubiquitous aquatic bacteria, were isolated from the same samples. MICs were obtained applying a standardized agar dilution method. A markedly decreased susceptibility of F. psychrophilum isolates to most antimicrobial agents presently available for use in Danish aquaculture was detected, while the collected Y. ruckeri isolates remained largely sensitive to all therapeutic substances. Comparing the inlet and outlet samples, the increase of the antibiotic-resistant proportions observed among the culturable microflora was more pronounced and statistically significant among the motile aeromonads. High levels of individual and multiple antimicrobial resistances were demonstrated within the collected flavobacteria and aeromonads, thus indicating a substantial impact of fish farming on several groups of bacteria associated with aquacultural environments.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigbojlen 4, DK-1870 Frederiksberg C, Denmark. Phone: 45-35282710. Fax: 45-35282711. E-mail: ansc{at}kvl.dk.

Applied and Environmental Microbiology, November 2000, p. 4908-4915, Vol. 66, No. 11
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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