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Applied and Environmental Microbiology, December 2000, p. 5167-5173, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sequence Diversity of rulA among Natural Isolates of Pseudomonas syringae and Effect on Function of rulAB-Mediated UV Radiation Tolerance

George W. Sundin,1,* Janette L. Jacobs,1 and Jesús Murillo2

Department of Plant Pathology and Microbiology, Texas A&M University, College Station, Texas 77843-2132,1 and Instituto de Agrobiotecnologia y Recursos Naturales, CSIC-UPNA, Laboratorio de Patología Vegetal, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Pública de Navarra, 31006 Pamplona, Spain2

Received 19 April 2000/Accepted 13 September 2000

The rulAB locus confers tolerance to UV radiation and is borne on plasmids of the pPT23A family in Pseudomonas syringae. We sequenced 14 rulA alleles from P. syringae strains representing seven pathovars and found sequence differences of 1 to 12% within pathovar syringae, and up to 15% differences between pathovars. Since the sequence variation within rulA was similar to that of P. syringae chromosomal alleles, we hypothesized that rulAB has evolved over a long time period in P. syringae. A phylogenetic analysis of the deduced amino acid sequences of rulA resulted in seven clusters. Strains from the same plant host grouped together in three cases; however, strains from different pathovars grouped together in two cases. In particular, the rulA alleles from P. syringae pv. lachrymans and P. syringae pv. pisi were grouped but were clearly distinct from the other sequenced alleles, suggesting the possibility of a recent interpathovar transfer. We constructed chimeric rulAB expression clones and found that the observed sequence differences resulted in significant differences in UV (wavelength) radiation sensitivity. Our results suggest that specific amino acid changes in RulA could alter UV radiation tolerance and the competitiveness of the P. syringae host in the phyllosphere.

* Corresponding author. Mailing address: Department of Plant Pathology and Microbiology, Texas A&M University, 2132 TAMU, College Station, TX 77843-2132. Phone: (979) 862-7518. Fax: (979) 845-6483. E-mail: gsundin{at}acs.tamu.edu.

Applied and Environmental Microbiology, December 2000, p. 5167-5173, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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