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Applied and Environmental Microbiology, December 2000, p. 5226-5230, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effect of Linoleic Acid Concentration on Conjugated Linoleic Acid Production by Butyrivibrio fibrisolvens A38

Young Jun Kim,1 Rui Hai Liu,1 Daniel R. Bond,2 and James B. Russell2,3,*

Departments of Food Science1 and Microbiology,2 Cornell University, and Agricultural Research Service, U.S. Department of Agriculture,3 Ithaca, New York 14853

Received 15 May 2000/Accepted 22 September 2000

Butyrivibrio fibrisolvens A38 inocula were inhibited by as little as 15 µM linoleic acid (LA), but growing cultures tolerated 10-fold more LA before growth was inhibited. Growing cultures did not produce significant amounts of cis-9, trans-11 conjugated linoleic acid (CLA) until the LA concentration was high enough to inhibit biohydrogenation, growth was inhibited, and lysis was enhanced. Washed-cell suspensions that were incubated anaerobically with 350 µM LA converted most of the LA to hydrogenated products, and little CLA was detected. When the washed-cell suspensions were incubated aerobically, biohydrogenation was inhibited, CLA production was at least twofold greater, and CLA persisted. The LA isomerase reaction was very rapid, but the LA isomerase did not recycle like a normal enzyme to catalyze more substrate. Cells that were preincubated with CLA lost their ability to produce more CLA from LA, and the CLA accumulation was directly proportional (r2 = 0.98) to the initial cell density. Growing cells were as sensitive to CLA as LA, the LA isomerase and reductases of biohydrogenation were linked, and free CLA was not released. Because growing cultures of B. fibrisolvens A38 did not produce significant amounts of CLA until the LA concentration was high, biohydrogenation was arrested, and the cell density had declined, the flow of CLA from the rumen may be due to LA-dependent bacterial inactivation, death, or lysis.

* Corresponding author. Mailing address: Cornell University, Wing Hall, Ithaca, NY 14853. Phone: (607) 255-4508. Fax: (607) 255-3904. E-mail: jbr8{at}cornell.edu.

Applied and Environmental Microbiology, December 2000, p. 5226-5230, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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