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Applied and Environmental Microbiology, December 2000, p. 5383-5386, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Construction and Characterization of an Escherichia coli Strain Genetically Engineered for Ni(II) Bioaccumulation

Rahul Krishnaswamy1 and David B. Wilson2,*

Field of Environmental Toxicology1 and Department of Molecular Biology and Genetics,2 Cornell University, Ithaca, New York 14853

Received 19 May 2000/Accepted 22 September 2000

An Escherichia coli strain that accumulated Ni(II) was constructed by introducing the nixA gene (coding for a nickel transport system) from Helicobacter pylori into JM109 cells that expressed a glutathione S-transferase-pea metallothionein fusion protein. The resulting strain accumulated 15 µmol of Ni(II) per g (dry weight) from a 10 µM Ni(II) solution, four times the level taken up by JM109 cells. Ni(II) accumulation did not require an energy source, was inhibited by only 50% by 0.1 M NaCl, and occurred over the pH range from 3 to 9.

* Corresponding author. Mailing address: Department of Molecular Biology & Genetics, Cornell University, 458 Biotechnology Building, Ithaca, NY 14853. Phone: (607) 255-5706. Fax: (607) 255-6249. E-mail: dbw3{at}cornell.edu.

Applied and Environmental Microbiology, December 2000, p. 5383-5386, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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