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Applied and Environmental Microbiology, December 2000, p. 5480-5483, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning, Nucleotide Sequence, and Expression of the Gene Encoding the Bacteriocin Boticin B from Clostridium botulinum Strain 213B

Sean S. Dineen, Marite Bradshaw, and Eric A. Johnson*

Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin, Madison, Wisconsin 53706

Received 26 June 2000/Accepted 19 September 2000

Boticin B is a heat-stable bacteriocin produced by Clostridium botulinum strain 213B that has inhibitory activity against various strains of C. botulinum and related clostridia. The gene encoding the bacteriocin was localized to a 3.0-kb HindIII fragment of an 18.8-kb plasmid, cloned, and sequenced. DNA sequencing revealed the boticin B structural gene, btcB, to be an open reading frame encoding 50 amino acids. A C. botulinum strain 62A transconjugant containing the HindIII fragment inserted into a clostridial shuttle vector expressed boticin B, although at much lower levels than those observed in C. botulinum 213B. To our knowledge, this is the first demonstration and characterization of a bacteriocin from toxigenic group I C. botulinum.

* Corresponding author. Mailing address: Department of Food Microbiology and Toxicology, Food Research Institute, University of Wisconsin, 1925 Willow Dr., Madison, WI 53706-1187. Phone: (608) 263-7944. Fax: (608) 263-1114. E-mail: eajohnso{at}facstaff.wisc.edu.

Applied and Environmental Microbiology, December 2000, p. 5480-5483, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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