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Applied and Environmental Microbiology, December 2000, p. 5484-5487, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Osmotic Stress-Induced Genetic Rearrangements in Escherichia coli H10407 Detected by Randomly Amplified Polymorphic DNA Analysis

Anne Jolivet-Gougeon,1,* Sandrine David-Jobert,1 Zohreh Tamanai-Shacoori,1 Christian Ménard,2 and Michel Cormier1

Laboratoire de Microbiologie Pharmaceutique, Université de Rennes I, 35000 Rennes, France,1 and Centre de Recherche en Infectiologie de l'Université Laval, CHUQ, Sainte-Foy, Quebec, Canada, G1V 4G22

Received 24 April 2000/Accepted 17 August 2000

Randomly amplified polymorphic DNA (RAPD) analysis is a DNA polymorphism assay commonly used for fingerprinting genomes. After optimizing the reaction conditions, samples of Escherichia coli H10407 DNA were assayed to determine the influence of osmotic and/or oligotrophic stress on variations in RAPD banding patterns. Genetic rearrangements or DNA topology variations could be detected as changes in agarose gel electrophoresis banding profiles. A new amplicon generated using DNA extracted from bacteria prestarved by an osmotic stress and resuscitated in rich medium was observed. Enrichment improved recovery of mutator cells and allowed them to be detected in samples, suggesting that DNA modifications, such as stress-induced alterations and supercoiling phenomena, should be taken into consideration before beginning RAPD analyses.

* Corresponding author. Mailing address: Laboratoire de Microbiologie Pharmaceutique, Université de Rennes I, 2 avenue du Professeur Léon Bernard, 35000 Rennes, France. Phone: (33) 02 99 33 69 16. Fax: (33) 02 99 33 62 60. E-mail: Anne.Gougeon{at}univ-rennes1.fr.

Applied and Environmental Microbiology, December 2000, p. 5484-5487, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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