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Applied and Environmental Microbiology, December 2000, p. 5499-5502, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sequence Differences in the Diagnostic Target Region of the Oocyst Wall Protein Gene of Cryptosporidium Parasites

Lihua Xiao,1,* Josef Limor,1 Una M. Morgan,2 Irshad M. Sulaiman,1 R. C. Andrew Thompson,2 and Altaf A. Lal1

Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30341,1 and State Agricultural Biotechnological Centre, Murdoch University, Murdoch, Western Australia 6150, Australia2

Received 6 July 2000/Accepted 22 September 2000

Nucleotide sequences of the Cryptosporidium oocyst wall protein (COWP) gene were obtained from various Cryptosporidium spp. (C. wrairi, C. felis, C. meleagridis, C. baileyi, C. andersoni, C. muris, and C. serpentis) and C. parvum genotypes (human, bovine, monkey, marsupial, ferret, mouse, pig, and dog). Significant diversity was observed among species and genotypes in the primer and target regions of a popular diagnostic PCR. These results provide useful information for COWP-based molecular differentiation of Cryptosporidium spp. and genotypes.

* Corresponding author: Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Highway, Atlanta, GA 30341. Phone: (770) 488-4840. Fax: (770) 488-4454. E-mail: LAX0{at}CDC.GOV.

Applied and Environmental Microbiology, December 2000, p. 5499-5502, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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