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Applied and Environmental Microbiology, December 2000, p. 5506-5508, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Mating Type Assay for Fusarium circinatum

M. Margaret Wallace1 and Sarah F. Covert2,*

Department of Genetics1 and Warnell School of Forest Resources,2 University of Georgia, Athens, Georgia 30602-2152

Received 3 April 2000/Accepted 11 September 2000

A rapid and reliable mating type assay for Fusarium circinatum was created by applying primers specific for the MAT1-1 and MAT1-2 mating type alleles to genomic DNA in a single PCR. A similar approach may be applied to fungi not previously shown to reproduce sexually, thus enabling studies of population structure and inheritance.

* Corresponding author. Mailing address: Warnell School of Forest Resources, University of Georgia, Athens, GA 30602-2152. Phone: (706) 542-1205. Fax: (706) 542-8356. E-mail: covert{at}arches.uga.edu.

Applied and Environmental Microbiology, December 2000, p. 5506-5508, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kerenyi, Z., Moretti, A., Waalwijk, C., Olah, B., Hornok, L. (2004). Mating Type Sequences in Asexually Reproducing Fusarium Species. Appl. Environ. Microbiol. 70: 4419-4423 [Abstract] [Full Text]  
  • Schweigkofler, W., O'Donnell, K., Garbelotto, M. (2004). Detection and Quantification of Airborne Conidia of Fusarium circinatum, the Causal Agent of Pine Pitch Canker, from Two California Sites by Using a Real-Time PCR Approach Combined with a Simple Spore Trapping Method. Appl. Environ. Microbiol. 70: 3512-3520 [Abstract] [Full Text]  


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