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Applied and Environmental Microbiology, December 2000, p. 5509-5513, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effect of Calcium in Assay Medium on D Value of Bacillus stearothermophilus ATCC 7953 Spores

Koichi Sasaki,1,* Hideharu Shintani,2 Junpei Itoh,3 Takuji Kamogawa,1 and Yousei Kajihara4

Quality Control Department, Misato Plant, Eisai Co., Ltd., Misato-Machi, Kodama-Gun, Saitama 367-0198,1 Division of Medical Devices, National Institute of Health Sciences, Setagaya-ku, Tokyo 158-0098,2 PPM Manufacturing, Fukushima Plant, Nippon Becton Dickinson Company, Ltd., Tsuchifune, Fukushima City, Fukushima 960-2152,3 and Life Tech Division, Nihon Pharmaceutical Co., Ltd., Sumiyoshi-cho, Izumisano City, Osaka 598-8558,4 Japan

Received 24 May 2000/Accepted 29 September 2000

The D value of commercial biological indicator spore strips using Bacillus stearothermophilus ATCC 7953 was increased by higher calcium concentrations in assay media. The calcium concentration in assay media varied among the manufacturers. The calcium concentration in assay media is an important factor to consider to minimize the variation of D value.

* Corresponding author. Mailing address: Quality Control Department, Misato Plant, Eisai Co., Ltd., 950, Hiroki, Misato-Machi, Kodama-Gun, Saitama 367-0198, Japan. Phone: 81-495-76-4365. Fax: 81-495-76-3942. E-mail: k-sasaki{at}hhc.eisai.co.jp.

Applied and Environmental Microbiology, December 2000, p. 5509-5513, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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