AEM
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by James, A. L.
Right arrow Articles by Gould, F. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by James, A. L.
Right arrow Articles by Gould, F. K.
Agricola
Right arrow Articles by James, A. L.
Right arrow Articles by Gould, F. K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, December 2000, p. 5521-5523, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of p-Naphtholbenzein-beta -D-Galactoside as a Substrate for Bacterial beta -Galactosidase

A. L. James,1 K. F. Chilvers,2,* J. D. Perry,2 L. Armstrong,1 and F. K. Gould2

Department of Chemical & Life Sciences, University of Northumbria,1 and Department of Microbiology, Freeman Hospital,2 Newcastle-upon-Tyne, United Kingdom

Received 15 February 2000/Accepted 3 October 2000

We describe the synthesis of a new substrate for the detection of beta -galactosidase and evaluate its performance in comparison with 5-bromo-4-chloro-3-indolyl-beta -D-galactopyranoside (X-Gal) and cyclohexenoesculetinbeta -D-galactoside (CHE-Gal). Of 206 Enterobacteriaceae strains able to hydrolyze X-Gal, 194 (94.2%) hydrolyzed CHE-Gal and 192 (93.2%) hydrolyzed p-naphtholbenzein-beta -D-galactoside (PNB-Gal). We conclude that PNB-Gal is an effective substrate for the detection of beta -galactosidase.

* Corresponding author. Mailing address: Department of Microbiology, Freeman Hospital, High Heaton, Newcastle-upon-Tyne NE7 7DN, United Kingdom. Phone: 0191-2843111, ext. 26291. Fax: 0191-2231226. E-mail: homeresearch{at}compuserve.com.

Applied and Environmental Microbiology, December 2000, p. 5521-5523, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. Microbiol. Mol. Biol. Rev. Eukaryot. Cell All ASM Journals

Copyright © 2000 by the American Society for Microbiology. All rights reserved.