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Applied and Environmental Microbiology, December 2000, p. 5524-5526, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Bacterial Toxin-Antitoxin Gene System as Containment Control in Yeast Cells

P. Kristoffersen,1 G. B. Jensen,2 K. Gerdes,3 and J. Piskur1,*

Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby,1 National Institute of Occupational Health, DK-2100 Copenhagen Ø,2 and Department of Molecular Biology, Odense University, DK-5230 Odense M,3 Denmark

Received 26 June 2000/Accepted 29 September 2000

The potential of a bacterial toxin-antitoxin gene system for use in containment control in eukaryotes was explored. The Escherichia coli relE and relB genes were expressed in the yeast Saccharomyces cerevisiae. Expression of the relE gene was highly toxic to yeast cells. However, expression of the relB gene counteracted the effect of relE to some extent, suggesting that toxin-antitoxin interaction also occurs in S. cerevisiae. Thus, bacterial toxin-antitoxin gene systems also have potential applications in the control of cell proliferation in eukaryotic cells, especially in those industrial fermentation processes in which the escape of genetically modified cells would be considered highly risky.

* Corresponding author. Mailing address: Department of Microbiology, Technical University of Denmark, Building 301, DK-2800 Lyngby, Denmark. Phone: (45) 4525 2518. Fax: (45) 4593 2809. E-mail: imjp{at}pop.dtu.dk.

Applied and Environmental Microbiology, December 2000, p. 5524-5526, Vol. 66, No. 12
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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