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Applied and Environmental Microbiology, February 2000, p. 599-605, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genetically Modified Vibrio harveyi Strains as Potential Bioindicators of Mutagenic Pollution of Marine Environments

Agata Czyz,1,2 Jacek Jasiecki,1 Adam Bogdan,1,3,dagger Hanna Szpilewska,3 and Grzegorz Wegrzyn1,*

Department of Molecular Biology, University of Gdansk1 and Laboratory of Molecular Biology affiliated with University of Gdansk,2 Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 80-822 Gdansk, and Marine Biology Center, Polish Academy of Sciences, 81-847 Gdynia,3 Poland

Received 19 August 1999/Accepted 19 November 1999

For biodetection of mutagenic pollution of marine environments, an organism naturally occurring in these habitats should be used. We found that marine bacterium Vibrio harveyi may be an appropriate bioindicator of mutagenic pollution. For positive selection of mutants, we developed a simple method for isolation of V. harveyi mutants resistant to neomycin. We constructed genetically modified V. harveyi strains that produce significantly more neomycin-resistant mutants upon treatment with low concentrations of mutagens than the wild-type counterpart. The sensitivity of the mutagenicity test with the V. harveyi strains is at least comparable to (if not higher than) that of the commonly used Ames test, which uses Salmonella enterica serovar Typhimurium strains. Therefore, we consider that the V. harveyi strains described in this report could be used as potential bioindicators of mutagenic pollution of marine environments.

* Corresponding author. Mailing address: Department of Molecular Biology, University of Gdansk, Kładki 24, 80-822 Gdansk, Poland. Phone: 48 (58) 346 3014. Fax: 48 (58) 301 0072. E-mail: wegrzyn{at}biotech.univ.gda.pl.

dagger Present address: Department of Biology and Pharmaceutical Botany, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdansk, Poland.

Applied and Environmental Microbiology, February 2000, p. 599-605, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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