This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Stevenson, T. H.
Right arrow Articles by Acuff, G. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stevenson, T. H.
Right arrow Articles by Acuff, G. R.
Agricola
Right arrow Articles by Stevenson, T. H.
Right arrow Articles by Acuff, G. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2000, p. 723-727, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development of a Selective Medium for Isolation of Helicobacter pylori from Cattle and Beef Samples

Timothy H. Stevenson, Lisa M. Lucia, and Gary R. Acuff*

Department of Animal Science, Texas A&M University, College Station, Texas 77843

Received 6 July 1999/Accepted 1 November 1999

Helicobacter pylori has been isolated from the human stomach with media containing only minimal selective agents. However, current research on the transmission and sources of infection requires more selective media due to the higher numbers of contaminants in environmental, oral, and fecal samples. The objective of this study was to develop and evaluate detection techniques that are sufficiently selective to isolate H. pylori from potential animal and food sources. Since H. pylori survives in the acidic environment of the stomach, low pH with added urea was studied as a potential selective combination. H. pylori grew fairly well on H. pylori Special Peptone plating medium supplemented with 10 mM urea at pH 4.5, but this pH did not sufficiently inhibit the growth of contaminants. Various antibiotic combinations were then compared, and a combination consisting of 10 mg of vancomycin per liter, 5 mg of amphotericin B per liter, 10 mg of cefsulodin per liter, 62,000 IU of polymyxin B sulfate per liter, 40 mg of trimethoprim per liter, and 20 mg of sulfamethoxazole per liter proved to be highly selective but still allowed robust colonies of H. pylori to grow. This medium was highly selective for recovering H. pylori from cattle and beef samples, and it is possible that it could be used to enhance the recovery of this bacterium from human and environmental samples, which may be contaminated with large numbers of competing microorganisms.

* Corresponding author. Mailing address: Department of Animal Science, 310 Kleberg, Texas A & M University, College Station, TX 77843-2471. Phone: (409) 845-4425. Fax: (409) 862-3475. E-mail: gacuff{at}tamu.edu.

Applied and Environmental Microbiology, February 2000, p. 723-727, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Azevedo, N. F., Pinto, A. R., Reis, N. M., Vieira, M. J., Keevil, C. W. (2006). Shear Stress, Temperature, and Inoculation Concentration Influence the Adhesion of Water-Stressed Helicobacter pylori to Stainless Steel 304 and Polypropylene. Appl. Environ. Microbiol. 72: 2936-2941 [Abstract] [Full Text]  
  • Lin, Y. T., Kwon, Y. I., Labbe, R. G., Shetty, K. (2005). Inhibition of Helicobacter pylori and Associated Urease by Oregano and Cranberry Phytochemical Synergies. Appl. Environ. Microbiol. 71: 8558-8564 [Abstract] [Full Text]  
  • Azevedo, N. F., Pacheco, A. P., Keevil, C. W., Vieira, M. J. (2004). Nutrient Shock and Incubation Atmosphere Influence Recovery of Culturable Helicobacter pylori from Water. Appl. Environ. Microbiol. 70: 490-493 [Abstract] [Full Text]  
  • Degnan, A. J., Sonzogni, W. C., Standridge, J. H. (2003). Development of a Plating Medium for Selection of Helicobacter pylori from Water Samples. Appl. Environ. Microbiol. 69: 2914-2918 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»