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Applied and Environmental Microbiology, February 2000, p. 825-827, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning and Sequencing of an Alkaline Protease Gene from Bacillus lentus and Amplification of the Gene on the B. lentus Chromosome by an Improved Technique

Per Linå Jørgensen,1 Martin Tangney,2 Poul Erik Pedersen,1 Sven Hastrup,1 Børge Diderichsen,1 and Steen T. Jørgensen1,*

Bacterial Gene Technology, Novo Nordisk A/S, 2880 Bagsværd, Copenhagen, Denmark,1 and Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland, United Kingdom2

Received 25 August 1999/Accepted 19 November 1999

A gene encoding an alkaline protease was cloned from an alkalophilic bacillus, and its nucleotide sequence was determined. The cloned gene was used to increase the copy number of the protease gene on the chromosome by an improved gene amplification technique.

* Corresponding author. Mailing address: Bacterial Gene Technology, Novo Nordisk A/S, 2880 Bagsværd, Copenhagen, Denmark. Phone: 45 4442 2809. Fax: 45 4442 7303. E-mail: stjq{at}novo.dk.

Applied and Environmental Microbiology, February 2000, p. 825-827, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




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