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Applied and Environmental Microbiology, February 2000, p. 828-831, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Phosphate Inhibits Acetotrophic Methanogenesis on Rice Roots

Ralf Conrad,* Melanie Klose, and Peter Claus

Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany

Received 10 September 1999/Accepted 22 November 1999

The contribution of acetate- and H2/CO2-dependent methanogenesis to total CH4 production was determined in excised washed rice roots by radiolabeling, methyl fluoride inhibition, and stable carbon isotope fractionation. Addition of >= 20 mM phosphate inhibited methanogenesis, which then was exclusively from H2/CO2. Otherwise, acetate contributed about 50 to 60% of the total methanogenesis, demonstrating that phosphate specifically inhibited acetotrophic methanogens on rice roots.

* Corresponding author. Mailing address: Max-Planck-Institut für terrestrische Mikrobiologie, Karl-von-Frisch-Str., D-35043 Marburg, Germany. Phone: 49 (6421) 178 801. Fax: 49 (6421) 178 809. E-mail: conrad{at}mailer.uni-marburg.de.

Applied and Environmental Microbiology, February 2000, p. 828-831, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Hori, T., Noll, M., Igarashi, Y., Friedrich, M. W., Conrad, R. (2007). Identification of Acetate-Assimilating Microorganisms under Methanogenic Conditions in Anoxic Rice Field Soil by Comparative Stable Isotope Probing of RNA. Appl. Environ. Microbiol. 73: 101-109 [Abstract] [Full Text]  
  • Lueders, T., Pommerenke, B., Friedrich, M. W. (2004). Stable-Isotope Probing of Microorganisms Thriving at Thermodynamic Limits: Syntrophic Propionate Oxidation in Flooded Soil. Appl. Environ. Microbiol. 70: 5778-5786 [Abstract] [Full Text]  


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