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Applied and Environmental Microbiology, February 2000, p. 832-835, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Influence of Osmotic and Nutritional Stress on Physiology of Penicillium fellutanum in Removal of Phosphocholine and Modification of Phospho-1-O-[N-Peptidyl-(2-Aminoethanol)] Phosphodiesters of Peptidophosphogalactomannan Speciesdagger

Yong-Il Park,Dagger Sandra J. Bonetti,§ and John E. Gander*

Department of Microbiology and Cell Science, University of Florida, Gainesville, Florida 32611-0700

Received 21 June 1999/Accepted 16 November 1999

Addition of 3 M NaCl to 72-h cultures of Penicillium fellutanum in 2 mM phosphate resulted in an increase in percentage of extracellular peptidophosphogalactomannan III (pPxGMiii) and a decrease in that of pPxGMii. The magnitude of 31P nuclear magnetic resonance signals at 1.47 and 1.33 ppm of phospho-1-O-[N-peptidyl-(2-aminoethanol)] phosphodiesters pPxGMii and pPxGMiii decreased compared with controls. The data suggest that serine, glycine, and threonine residues from the 3-kDa peptide and from galactofuranosyl-6-O-phospho-1'-O-[N-peptidyl-(2-aminoethanol)] residues were the precursors of the needed choline-derived osmolytes.

* Corresponding author. Mailing address: 4219 Rancho Grande Place N.W., Albuquerque, NM 87120-5337. Phone: (505) 898-4128. E-mail: jegander01{at}uswest.net.

dagger Journal Series number R06885 of the Florida Agricultural Experiment Station.

Dagger Present address: Environmental Bioresources Lab., Biotechnology Division, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Yusong, Taejon, 305-333 Korea.

§ Present address: Department of Chemistry, University of Southern Colorado, Pueblo, CO 81001-4901.

Applied and Environmental Microbiology, February 2000, p. 832-835, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Tuekam, B. A., Park, Y.-I., Unkefer, C. J., Gander, J. E. (2001). Relationship of Exo-{beta}-D-Galactofuranosidase Kinetic Parameters to the Number of Phosphodiesters in Penicillium fellutanum Peptidophosphogalactomannan: Enzyme Purification and Kinetics of Glycopeptide and Galactofuran Chain Hydrolysis. Appl. Environ. Microbiol. 67: 4648-4656 [Abstract] [Full Text]  


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