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Applied and Environmental Microbiology, February 2000, p. 836-838, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Effect of Catalase on Hydrogen Peroxide Penetration into Pseudomonas aeruginosa Biofilms

Philip S. Stewart,1,2,* Frank Roe,1 Joanna Rayner,1 James G. Elkins,1,3 Zbigniew Lewandowski,1,4 Urs A. Ochsner,5 and Daniel J. Hassett6

Center for Biofilm Engineering,1 Department of Chemical Engineering,2 Department of Land Resources and Environmental Science,3 and Department of Civil Engineering,4 Montana State University---Bozeman, Bozeman, Montana 59717-3980; Department of Microbiology, University of Colorado Health Sciences Center, Denver, Colorado 802625; and Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati College of Medicine, Cincinnati, Ohio 45257-05246

Received 24 June 1999/Accepted 9 November 1999

The penetration of hydrogen peroxide into biofilms formed by wild-type and catalase-deficient Pseudomonas aeruginosa strains was measured using microelectrodes. A flowing stream of hydrogen peroxide (50 mM, 1 h) was unable to penetrate or kill wild-type biofilms but did penetrate and partially kill biofilms formed by an isogenic strain in which the katA gene was knocked out. Catalase protects aggregated bacteria by preventing full penetration of hydrogen peroxide into the biofilm.

* Corresponding author. Mailing address: Center for Biofilm Engineering, 366 EPS Building, Montana State University, Bozeman, MT 59717-3980. Phone: (406) 994-2890. Fax: (406) 994-6098. E-mail: phil_s{at}erc.montana.edu.

Applied and Environmental Microbiology, February 2000, p. 836-838, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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