This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gavin, J.
Right arrow Articles by Logan, N. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gavin, J.
Right arrow Articles by Logan, N. A.
Agricola
Right arrow Articles by Gavin, J.
Right arrow Articles by Logan, N. A.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, February 2000, p. 874-875, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Observation of Soft Contact Lens Disinfection with Fluorescent Metabolic Stains

J. Gavin,1,2 N. F. Button,2 I. A. Watson-Craik,3 and N. A. Logan1,*

School of Biological and Biomedical Sciences1 and Department of Vision Sciences,2 Glasgow Caledonian University, Glasgow G4 OBA, and Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow G1 1XW,3 United Kingdom

Received 1 June 1999/Accepted 19 November 1999

A rapid fluorescent staining method using a tetrazolium dye and propidium iodide for the in-use assessment of disinfection of Pseudomonas aeruginosa biofilms on soft contact lenses showed that 11 to 13% of cells on lenses remained actively respiring and recoverable by culture methods after 30 min of exposure to 3% hydrogen peroxide.

* Corresponding author. Mailing address: School of Biological and Biomedical Sciences and Department of Vision Sciences, Glasgow Caledonian University, Cowcaddens Rd., Glasgow G4 OBA, United Kingdom. Phone: (44) 141 331 3207. Fax: (44) 141 331 3208. E-mail: N.A.Logan{at}gcal.ac.uk.

Applied and Environmental Microbiology, February 2000, p. 874-875, Vol. 66, No. 2
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shin, D.-H., Choi, Y.-S., Cho, Y.-H. (2008). Unusual Properties of Catalase A (KatA) of Pseudomonas aeruginosa PA14 Are Associated with Its Biofilm Peroxide Resistance. J. Bacteriol. 190: 2663-2670 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»