This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kim, S.
Right arrow Articles by Ryu, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, S.
Right arrow Articles by Ryu, S.
Agricola
Right arrow Articles by Kim, S.
Right arrow Articles by Ryu, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, March 2000, p. 1213-1215, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Inhibitory Effects of Collagen on the PCR for Detection of Clostridium perfringens

Sangburm Kim,1 Ronald G. Labbe,2 and Sangryeol Ryu1,*

Department of Food Science and Technology, Seoul National University, Suwon, Korea,1 and Department of Food Science, University of Massachusetts, Amherst, Massachusetts2

Received 27 October 1999/Accepted 10 November 1999

It is essential to identify specific food components that inhibit PCR in order to increase the sensitivity of the PCR method for rapid detection of pathogens contaminating a food. We found that collagen, a major component of several foods, inhibited PCR. The inhibitory action of collagen on PCR could be partially reversed by adjusting the concentration of magnesium ion in the reaction mixture and by the use of various DNA extraction methods to remove the collagen from the DNA. Also, the source of thermostable DNA polymerase was affected by the presence of collagen. These results suggest the need to optimize the extraction and assay conditions for rapid detection of enterotoxigenic Clostridium perfringens by PCR with respect to the kind of food being analyzed.

* Corresponding author. Mailing address: Department of Food Science and Technology, Seoul National University, Suwon 441-744, Korea. Phone: 82-331-290-2584. Fax: 82-331-293-4789. E-mail: sangryu{at}snu.ac.kr.

Applied and Environmental Microbiology, March 2000, p. 1213-1215, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»