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Applied and Environmental Microbiology, March 2000, p. 1216-1219, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development of a Nonantibiotic Dominant Marker for Positively Selecting Expression Plasmids in Multivalent Salmonella Vaccines

Hesta V. McNeill, Katharine A. Sinha,dagger Carlos E. Hormaeche, Jeong Jin Lee, and C. M. Anjam Khan*

Department of Microbiology & Immunology, The Medical School, University of Newcastle, Newcastle NE2 4HH, United Kingdom

Received 20 April 1999/Accepted 10 December 1999

We report the novel application of a herbicide-resistance-based dominant marker for the positive selection of expression plasmids in Salmonella serovar vaccines. The beta -lactamase gene of the plasmid pTETnir15, which expresses fragment C of tetanus toxin (TetC), has been replaced with the bar gene marker. The new plasmid pBAT1 can be positively selected in vitro within Salmonella serovars in the presence of the herbicide DL-phosphinothricin. The expression of TetC remains unaltered, and the Salmonella enterica serovar Typhimurium vaccine strain is stable and immunogenic in vivo.

* Corresponding author. Mailing address: Department of Microbiology, The Medical School, University of Newcastle, Newcastle upon Tyne NE2 4HH, United Kingdom. Phone: 44-191-222-7066. Fax: 44-191-222-7736. E-mail: anjam.khan{at}newcastle.ac.uk.

dagger Present address: Department of Biochemistry, Imperial College, London SW7 2AZ, United Kingdom.

Applied and Environmental Microbiology, March 2000, p. 1216-1219, Vol. 66, No. 3
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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