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Applied and Environmental Microbiology, April 2000, p. 1523-1526, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Identification of Species from the Penicillium roqueforti Group Associated with Spoiled Animal Feed

Marianne E. Boysen,1 Karl-Gustav Jacobsson,2 and Johan Schnürer1,*

Department of Microbiology, Swedish University of Agricultural Sciences (SLU),1 and Feed Laboratory, National Veterinary Institute,2 SE-750 07 Uppsala, Sweden

Received 4 May 1999/Accepted 29 January 2000

The Penicillium roqueforti group has recently been split into three species, P. roqueforti, Penicillium carneum, and Penicillium paneum, on the basis of differences in ribosomal DNA sequences and secondary metabolite profiles. We reevaluated the taxonomic identity of 52 livestock feed isolates from Sweden, previously identified by morphology as P. roqueforti, by comparing the sequences of the ribosomal internal transcribed spacer region. Identities were confirmed with random amplified polymorphic DNA analysis and secondary metabolite profiles. Of these isolates, 48 were P. roqueforti, 2 were P. paneum, and 2 were Penicillium expansum. No P. carneum isolates were found. The three species produce different mycotoxins, but no obvious relationship between mold and animal disease was detected, based on medical records. P. roqueforti appears to dominate in silage, but the ecological and toxicological importance of P. carneum and P. paneum as feed spoilage fungi is not clear. This is the first report of P. expansum in silage.

* Corresponding author. Mailing address: Department of Microbiology, SLU, P.O. Box 7025, S-750 07 Uppsala, Sweden. Phone: 46 (0)18-67 32 15. Fax: 46 (0)18-67 33 92. E-mail: Johan.Schnurer{at}mikrob.slu.se.

Applied and Environmental Microbiology, April 2000, p. 1523-1526, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Mansfield, M.A., Kuldau, G.A. (2007). Microbiological and molecular determination of mycobiota in fresh and ensiled maize silage. Mycologia 99: 269-278 [Abstract] [Full Text]  
  • Rakeman, J. L., Bui, U., LaFe, K., Chen, Y.-C., Honeycutt, R. J., Cookson, B. T. (2005). Multilocus DNA Sequence Comparisons Rapidly Identify Pathogenic Molds. J. Clin. Microbiol. 43: 3324-3333 [Abstract] [Full Text]  


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