This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dojka, M. A.
Right arrow Articles by Pace, N. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dojka, M. A.
Right arrow Articles by Pace, N. R.
Agricola
Right arrow Articles by Dojka, M. A.
Right arrow Articles by Pace, N. R.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, April 2000, p. 1617-1621, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Expanding the Known Diversity and Environmental Distribution of an Uncultured Phylogenetic Division of Bacteria

Michael A. Dojka,dagger J. Kirk Harris, and Norman R. Pace*

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347

Received 29 November 1999/Accepted 23 January 2000

Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated sequences were cloned from 7 of 12 environments investigated: mainly from anaerobic sediment environments. The number of sequences representing the WS6 candidate division was increased from 3 to 60 in this study. The extent of phylogenetic divergence (sequence difference) in this candidate division was found to be among the largest of any known bacterial division. This indicates that organisms representing the WS6 phylogenetic division offer a broad diversity of undiscovered biochemical and metabolic novelty. These results provide a framework for the further study of these evidently important kinds of organisms and tools, the sequences, with which to do so.

* Corresponding author. Mailing address: Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, CO 80309-0347. Phone: (303) 735-1864. Fax: (303) 492-7744. E-mail: nrpace{at}colorado.edu.

dagger Present address: Maxygen, Inc., Redwood City, CA 94063.

Applied and Environmental Microbiology, April 2000, p. 1617-1621, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Davis, J. P., Youssef, N. H., Elshahed, M. S. (2009). Assessment of the Diversity, Abundance, and Ecological Distribution of Members of Candidate Division SR1 Reveals a High Level of Phylogenetic Diversity but Limited Morphotypic Diversity. Appl. Environ. Microbiol. 75: 4139-4148 [Abstract] [Full Text]  
  • Robertson, C. E., Spear, J. R., Harris, J. K., Pace, N. R. (2009). Diversity and Stratification of Archaea in a Hypersaline Microbial Mat. Appl. Environ. Microbiol. 75: 1801-1810 [Abstract] [Full Text]  
  • Lee, P. K. H., Macbeth, T. W., Sorenson, K. S. Jr., Deeb, R. A., Alvarez-Cohen, L. (2008). Quantifying Genes and Transcripts To Assess the In Situ Physiology of "Dehalococcoides" spp. in a Trichloroethene-Contaminated Groundwater Site. Appl. Environ. Microbiol. 74: 2728-2739 [Abstract] [Full Text]  
  • Lee, P. K. H., Johnson, D. R., Holmes, V. F., He, J., Alvarez-Cohen, L. (2006). Reductive Dehalogenase Gene Expression as a Biomarker for Physiological Activity of Dehalococcoides spp.. Appl. Environ. Microbiol. 72: 6161-6168 [Abstract] [Full Text]  
  • Janssen, P. H. (2006). Identifying the Dominant Soil Bacterial Taxa in Libraries of 16S rRNA and 16S rRNA Genes.. Appl. Environ. Microbiol. 72: 1719-1728 [Full Text]  
  • Chouari, R., Le Paslier, D., Dauga, C., Daegelen, P., Weissenbach, J., Sghir, A. (2005). Novel Major Bacterial Candidate Division within a Municipal Anaerobic Sludge Digester. Appl. Environ. Microbiol. 71: 2145-2153 [Abstract] [Full Text]  
  • Webster, G., Parkes, R. J., Fry, J. C., Weightman, A. J. (2004). Widespread Occurrence of a Novel Division of Bacteria Identified by 16S rRNA Gene Sequences Originally Found in Deep Marine Sediments. Appl. Environ. Microbiol. 70: 5708-5713 [Abstract] [Full Text]  
  • Fieseler, L., Horn, M., Wagner, M., Hentschel, U. (2004). Discovery of the Novel Candidate Phylum "Poribacteria" in Marine Sponges. Appl. Environ. Microbiol. 70: 3724-3732 [Abstract] [Full Text]  
  • Zhou, J., Xia, B., Huang, H., Palumbo, A. V., Tiedje, J. M. (2004). Microbial Diversity and Heterogeneity in Sandy Subsurface Soils. Appl. Environ. Microbiol. 70: 1723-1734 [Abstract] [Full Text]  
  • Harris, J. K., Kelley, S. T., Pace, N. R. (2004). New Perspective on Uncultured Bacterial Phylogenetic Division OP11. Appl. Environ. Microbiol. 70: 845-849 [Abstract] [Full Text]  
  • Chouari, R., Le Paslier, D., Daegelen, P., Ginestet, P., Weissenbach, J., Sghir, A. (2003). Molecular Evidence for Novel Planctomycete Diversity in a Municipal Wastewater Treatment Plant. Appl. Environ. Microbiol. 69: 7354-7363 [Abstract] [Full Text]  
  • Zhang, H., Sekiguchi, Y., Hanada, S., Hugenholtz, P., Kim, H., Kamagata, Y., Nakamura, K. (2003). Gemmatimonas aurantiaca gen. nov., sp. nov., a Gram-negative, aerobic, polyphosphate-accumulating micro-organism, the first cultured representative of the new bacterial phylum Gemmatimonadetes phyl. nov.. Int. J. Syst. Evol. Microbiol. 53: 1155-1163 [Abstract] [Full Text]  
  • Stoeck, T., Epstein, S. (2003). Novel Eukaryotic Lineages Inferred from Small-Subunit rRNA Analyses of Oxygen-Depleted Marine Environments. Appl. Environ. Microbiol. 69: 2657-2663 [Abstract] [Full Text]  
  • Liles, M. R., Manske, B. F., Bintrim, S. B., Handelsman, J., Goodman, R. M. (2003). A Census of rRNA Genes and Linked Genomic Sequences within a Soil Metagenomic Library. Appl. Environ. Microbiol. 69: 2684-2691 [Abstract] [Full Text]  
  • Bruns, A., Nubel, U., Cypionka, H., Overmann, J. (2003). Effect of Signal Compounds and Incubation Conditions on the Culturability of Freshwater Bacterioplankton. Appl. Environ. Microbiol. 69: 1980-1989 [Abstract] [Full Text]  
  • Frank, D. N., Spiegelman, G. B., Davis, W., Wagner, E., Lyons, E., Pace, N. R. (2003). Culture-Independent Molecular Analysis of Microbial Constituents of the Healthy Human Outer Ear. J. Clin. Microbiol. 41: 295-303 [Abstract] [Full Text]  
  • Lawrence, J. G., Hatfull, G. F., Hendrix, R. W. (2002). Imbroglios of Viral Taxonomy: Genetic Exchange and Failings of Phenetic Approaches. J. Bacteriol. 184: 4891-4905 [Abstract] [Full Text]  
  • Kaeberlein, T., Lewis, K., Epstein, S. S. (2002). Isolating "Uncultivable" Microorganisms in Pure Culture in a Simulated Natural Environment. Science 296: 1127-1129 [Abstract] [Full Text]  
  • Kashefi, K., Holmes, D. E., Reysenbach, A.-L., Lovley, D. R. (2002). Use of Fe(III) as an Electron Acceptor To Recover Previously Uncultured Hyperthermophiles: Isolation and Characterization of Geothermobacterium ferrireducens gen. nov., sp. nov.. Appl. Environ. Microbiol. 68: 1735-1742 [Abstract] [Full Text]  
  • Derakshani, M., Lukow, T., Liesack, W. (2001). Novel Bacterial Lineages at the (Sub)Division Level as Detected by Signature Nucleotide-Targeted Recovery of 16S rRNA Genes from Bulk Soil and Rice Roots of Flooded Rice Microcosms. Appl. Environ. Microbiol. 67: 623-631 [Abstract] [Full Text]  
  • Hugenholtz, P., Tyson, G. W., Webb, R. I., Wagner, A. M., Blackall, L. L. (2001). Investigation of Candidate Division TM7, a Recently Recognized Major Lineage of the Domain Bacteria with No Known Pure-Culture Representatives. Appl. Environ. Microbiol. 67: 411-419 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»