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Applied and Environmental Microbiology, April 2000, p. 1680-1684, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Engineering Desiccation Tolerance in Escherichia coli

Daniela Billi,1 Deborah J. Wright,1 Richard F. Helm,1 Todd Prickett,1 Malcolm Potts,1,* and John H. Crowe2

Virginia Tech Center for Genomics (VIGEN), Fralin Biotechnology Center, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061,1 and Department of Molecular and Cell Biology, University of California-Davis, Davis, California 956162

Received 12 October 1999/Accepted 16 January 2000

Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (P==O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.

* Corresponding author. Mailing address: VIGEN, Department of Biochemistry, 205 Engel Hall, W. Campus Drive, Virginia Tech, Blacksburg, VA 24061. Phone: (540) 231-5745. Fax: (540) 231-9070. E-mail: geordie{at}vt.edu.

Applied and Environmental Microbiology, April 2000, p. 1680-1684, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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