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Applied and Environmental Microbiology, April 2000, p. 1698-1701, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Tetrodotoxin-Producing Vibrio Strain, LM-1, from the Puffer Fish Fugu vermicularis radiatus

Myoung-Ja Lee,1,2,dagger Dong-Youn Jeong,2,dagger Woo-Seong Kim,2,dagger Hyun-Dae Kim,2,Dagger Cheorl-Ho Kim,1,* Won-Whan Park,1 Yong-Ha Park,3 Kyung-Sam Kim,2,§ Hyung-Min Kim,4 and Dong-Soo Kim2

Department of Diagnosis, Biochemistry and Molecular Biology, College of Oriental Medicine, Dongguk University, Kyung-Ju 780-714,1 KCTC, Korea Research Institute of Bioscience and Biotechnology, Taejon 305-600,3 College of Pharmacy, Wonkwang University, Iksan 570-749,4 and Department of Food Science and Technology, Kyungsung University, Pusan 608-736,2 Korea

Received 21 January 1999/Accepted 9 November 1999

Identification of tetrodotoxin (TTX) and its derivatives produced from a Vibrio strain in the intestine of the puffer fish Fugu vermicularis radiatus was performed by thin-layer chromatography, electrophoresis, high-performance liquid chromatography, and gas chromatography-mass spectrometry, together with a mouse bioassay for toxicity. It was demonstrated that the isolated bacterium produced TTX, 4-epi-TTX, and anhTTX during cultivation, suggesting that Vibrio strains are responsible for the toxification of the puffer fish.

* Corresponding author. Mailing address: Department of Diagnosis, Biochemistry, and Molecular Biology, College of Oriental Medicine, Dongguk University, Sukjang-Dong 707, Kyung-Ju 780-714, Korea. Phone: 82-561-770-2663. Fax: 82-561-770-2281. E-mail: chkimbio{at}mail.dongguk.ac.kr.

dagger Present address: Pusan Regional FDA, Pusan 608-080, Korea.

Dagger Present address: Department of Food Science and Nutrition, Dong-Pusan College, Pusan 612-715, Korea.

§ Present address: Department of Food Science and Nutrition, Pusan Women's College, Pusan 614-734, Korea.

Applied and Environmental Microbiology, April 2000, p. 1698-1701, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Ritson-Williams, R., Yotsu-Yamashita, M., Paul, V. J. (2006). Ecological functions of tetrodotoxin in a deadly polyclad flatworm. Proc. Natl. Acad. Sci. USA 103: 3176-3179 [Abstract] [Full Text]  
  • Matsumura, K., Kim, D.-S., Kim, C.-H. (2001). No Ability To Produce Tetrodotoxin in Bacteria. Appl. Environ. Microbiol. 67: 2393-2394 [Full Text]  


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