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Applied and Environmental Microbiology, April 2000, p. 1741-1743, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Cloning, Sequencing, and Expression in Escherichia coli of the New Gene Encoding beta -1,3-Xylanase from a Marine Bacterium, Vibrio sp. Strain XY-214

Toshiyoshi Araki,* Shinnosuke Hashikawa, and Tatsuo Morishita

Faculty of Bioresources, Mie University, Kamihama, Tsu, Mie 514-8507, Japan

Received 8 September 1999/Accepted 19 January 2000

The Vibrio sp. strain XY-214 beta -1,3-xylanase gene cloned in Escherichia coli DH5alpha consisted of an open reading frame of 1,383 nucleotides encoding a protein of 460 amino acids with a molecular mass of 51,323 Da and had a signal peptide of 22 amino acids. The transformant enzyme hydrolyzed beta -1,3-xylan to produce several xylooligosaccharides.

* Corresponding author. Mailing address: Faculty of Bioresources, Mie University, 1515 Kamihama, Tsu, Mie 514-8507, Japan.

Applied and Environmental Microbiology, April 2000, p. 1741-1743, Vol. 66, No. 4
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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