This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Laurie, A. D.
Right arrow Articles by Lloyd-Jones, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Laurie, A. D.
Right arrow Articles by Lloyd-Jones, G.
Agricola
Right arrow Articles by Laurie, A. D.
Right arrow Articles by Lloyd-Jones, G.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2000, p. 1814-1817, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Quantification of phnAc and nahAc in Contaminated New Zealand Soils by Competitive PCR

Andrew D. Lauriedagger and Gareth Lloyd-Jones*

Landcare Research, Hamilton, New Zealand

Received 7 September 1999/Accepted 7 February 2000

Unculturable polycyclic aromatic hydrocarbon (PAH)-degrading bacteria are a significant reservoir of the microbial potential to catabolize low-molecular-weight PAHs. The population of these bacteria is larger than the population of nah-like bacteria that are the dominant organisms in culture-based studies. We used the recently described phn genes of Burkholderia sp. strain RP007, which feature only rarely in culture-based studies, as an alternative genotype for naphthalene and phenanthrene degradation and compared this genotype with the genotypically distinct but ubiquitous nah-like class in different soils. Competitive PCR quantification of phnAc and nahAc, which encode the iron sulfur protein large (alpha ) subunits of PAH dioxygenases in nah-like and phn catabolic operons, revealed that the phn genotype can have a greater ecological significance than the nah-like genotype.

* Corresponding author. Mailing address: Landcare Research, Private Bag 3127, Hamilton, New Zealand. Phone: (64) 7 858 3700. Fax: (64) 7 858 4964. E-mail: lloyd-jonesg{at}landcare.cri.nz.

dagger Present address: Department for Cell and Molecular Biology, Umeå University, Umeå, Sweden.

Applied and Environmental Microbiology, May 2000, p. 1814-1817, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yagi, J. M., Madsen, E. L. (2009). Diversity, Abundance, and Consistency of Microbial Oxygenase Expression and Biodegradation in a Shallow Contaminated Aquifer. Appl. Environ. Microbiol. 75: 6478-6487 [Abstract] [Full Text]  
  • Singleton, D. R., Guzman Ramirez, L., Aitken, M. D. (2009). Characterization of a Polycyclic Aromatic Hydrocarbon Degradation Gene Cluster in a Phenanthrene-Degrading Acidovorax Strain. Appl. Environ. Microbiol. 75: 2613-2620 [Abstract] [Full Text]  
  • Gomes, N. C. M., Borges, L. R., Paranhos, R., Pinto, F. N., Krogerrecklenfort, E., Mendonca-Hagler, L. C. S., Smalla, K. (2007). Diversity of ndo Genes in Mangrove Sediments Exposed to Different Sources of Polycyclic Aromatic Hydrocarbon Pollution. Appl. Environ. Microbiol. 73: 7392-7399 [Abstract] [Full Text]  
  • Ni Chadhain, S. M., Norman, R. S., Pesce, K. V., Kukor, J. J., Zylstra, G. J. (2006). Microbial Dioxygenase Gene Population Shifts during Polycyclic Aromatic Hydrocarbon Biodegradation.. Appl. Environ. Microbiol. 72: 4078-4087 [Abstract] [Full Text]  
  • Xiao, X., Yin, X., Lin, J., Sun, L., You, Z., Wang, P., Wang, F. (2005). Chitinase Genes in Lake Sediments of Ardley Island, Antarctica. Appl. Environ. Microbiol. 71: 7904-7909 [Abstract] [Full Text]  
  • Dionisi, H. M., Chewning, C. S., Morgan, K. H., Menn, F.-M., Easter, J. P., Sayler, G. S. (2004). Abundance of Dioxygenase Genes Similar to Ralstonia sp. Strain U2 nagAc Is Correlated with Naphthalene Concentrations in Coal Tar-Contaminated Freshwater Sediments. Appl. Environ. Microbiol. 70: 3988-3995 [Abstract] [Full Text]  
  • Van Hamme, J. D., Singh, A., Ward, O. P. (2003). Recent Advances in Petroleum Microbiology. Microbiol. Mol. Biol. Rev. 67: 503-549 [Abstract] [Full Text]  
  • Wilson, M. S., Herrick, J. B., Jeon, C. O., Hinman, D. E., Madsen, E. L. (2003). Horizontal Transfer of phnAc Dioxygenase Genes within One of Two Phenotypically and Genotypically Distinctive Naphthalene-Degrading Guilds from Adjacent Soil Environments. Appl. Environ. Microbiol. 69: 2172-2181 [Abstract] [Full Text]  
  • Park, W., Padmanabhan, P., Padmanabhan, S., Zylstra, G. J., Madsen, E. L. (2002). nahR, encoding a LysR-type transcriptional regulator, is highly conserved among naphthalene-degrading bacteria isolated from a coal tar waste-contaminated site and in extracted community DNA. Microbiology 148: 2319-2329 [Abstract] [Full Text]  
  • Abed, R. M. M., Safi, N. M. D., Koster, J., de Beer, D., El-Nahhal, Y., Rullkotter, J., Garcia-Pichel, F. (2002). Microbial Diversity of a Heavily Polluted Microbial Mat and Its Community Changes following Degradation of Petroleum Compounds. Appl. Environ. Microbiol. 68: 1674-1683 [Abstract] [Full Text]  
  • Lim, E. L., Tomita, A. V., Thilly, W. G., Polz, M. F. (2001). Combination of Competitive Quantitative PCR and Constant-Denaturant Capillary Electrophoresis for High-Resolution Detection and Enumeration of Microbial Cells. Appl. Environ. Microbiol. 67: 3897-3903 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»