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Applied and Environmental Microbiology, May 2000, p. 1851-1856, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of an Enzyme-Linked Lectinsorbent Assay To Monitor the Shift in Polysaccharide Composition in Bacterial Biofilms

V. Leriche,1,2 P. Sibille,3 and B. Carpentier2,*

SODIAAL UNION, Paris,1 Laboratoire d'Études et de Recherches pour l'Alimentation Collective, Agence Française de Sécurité Sanitaire des Aliments, Maisons-Alfort,2 and Unité de Pathologie Aviaire et Parasitologie, Institut National de la Recherche Agronomique, Centre de Tours,3 France

Received 18 October 1999/Accepted 18 February 2000

An enzyme-linked lectinsorbent assay (ELLA) was developed for quantification and characterization of extracellular polysaccharides produced by 1- and 4-day biofilms of 10 bacterial strains isolated from food industry premises. Peroxidase-labeled concanavalin A (ConA) and wheat germ agglutinin (WGA) were used, as they specifically bind to saccharide residues most frequently encountered in biofilms matrices: D-glucose or D-mannose for ConA and N-acetyl-D-glucosamine or N-acetylneuraminic acid for WGA. The ELLA applied to 1- and 4-day biofilms colonizing wells of microtiter plates was able to detect that for Stenotrophomonas maltophilia and to a lesser extent Staphylococcus sciuri, the increase in production of exopolysaccharides over time was not the same for sugars binding with ConA and those binding with WGA. Differences in extracellular polysaccharides produced were observed among strains belonging to the same species. These results demonstrate that ELLA is a useful tool not only for rapid characterization of biofilm extracellular polysaccharides but also, in studies of individual strains, for detection of changes over time in the proportion of the exopolysaccharidic component within the polymeric matrix.

* Corresponding author. Mailing address: AFSSA Lerpac, 22 rue Pierre Curie, B.P. 332, F-94709 Maisons Alfort Cedex, France. Phone: 33(0)1-49 77 26 46. Fax: 33(0) 1-49 77 26 40. E-mail: b.carpentier{at}lerpac.afssa.fr.

Applied and Environmental Microbiology, May 2000, p. 1851-1856, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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