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Applied and Environmental Microbiology, May 2000, p. 1905-1910, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Enantiomeric Degradation of 2-(4-Sulfophenyl)Butyrate via 4-Sulfocatechol in Delftia acidovorans SPB1

Saskia Schulz,1,2,dagger Wenbo Dong,1,Dagger Ulrich Groth,2 and Alasdair M. Cook1,*

Departments of Biology1 and Chemistry,2 The University of Konstanz, D-78457 Konstanz, Germany

Received 2 September 1999/Accepted 7 February 2000

Enrichment cultures with enantiomeric 2-(4-sulfophenyl)butyrate (SPB) as the sole added source(s) of carbon and energy for growth yielded a pure culture of a degradative bacterium, which was identified as Delftia acidovorans SPB1. The organism utilized the enantiomers sequentially. R-SPB was utilized first (specific growth rate [µ] = 0.28 h-1), with transient excretion of an unknown intermediate, which was identified as 4-sulfocatechol (4SC). Utilization of S-SPB was slower (µ = 0.016 h-1) and was initiated only after the first enantiomer was exhausted. Suspensions of cells grown in S-SPB excreted 4SC, so metabolism of the two enantiomers converged at 4SC. The latter was degraded by ortho cleavage via 3-sulfo-cis,cis-muconate. Strain SPB1 grew with 4SC and with 1-(4-sulfophenyl)octane (referred to herein as model LAS) but not with commercial linear alkylbenzenesulfonate (LAS) surfactant, which is subterminally substituted but nontoxic. It would appear that metabolism of the model LAS does not represent metabolism of commercial LAS.

* Corresponding author. Mailing address: Department of Biology, The University of Konstanz, Universitätstr. 10, D-78457 Konstanz, Germany. Phone: (49) 7531 88 4247. Fax: (49) 7531 88 29 66. E-mail: Alasdair.Cook{at}uni-konstanz.de.

dagger Present address: Sartorius AG, D-37075 Göttingen, Germany.

Dagger Present address: Department of Environmental Science and Engineering, Fudan University, Shanghai, China.

Applied and Environmental Microbiology, May 2000, p. 1905-1910, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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