This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Manefield, M.
Right arrow Articles by Kjelleberg, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manefield, M.
Right arrow Articles by Kjelleberg, S.
Agricola
Right arrow Articles by Manefield, M.
Right arrow Articles by Kjelleberg, S.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2000, p. 2079-2084, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Inhibition of Luminescence and Virulence in the Black Tiger Prawn (Penaeus monodon) Pathogen Vibrio harveyi by Intercellular Signal Antagonists

Michael Manefield,1,* Lachlan Harris,2,* Scott A. Rice,1,3 Rocky de Nys,3,4 and Staffan Kjelleberg1,3

Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville,2 and School of Microbiology and Immunology,1 Centre for Marine Biofouling and Bio-Innovation3 and School of Biological Sciences,4 University of New South Wales, Sydney, Australia

Received 22 October 1999/Accepted 17 January 2000

Expression of luminescence in the Penaeus monodon pathogen Vibrio harveyi is regulated by an intercellular quorum sensing mechanism involving the synthesis and detection of two signaling molecules, one of which is N-hydroxy butanoyl-L-homoserine lactone and the other of which is uncharacterized. Indirect evidence has suggested that virulence, associated with a toxic extracellular protein, and luminescence in V. harveyi are coregulated. In this study the effects of an acylated homoserine lactone antagonist produced by the marine alga Delisea pulchra on luminescence and toxin production in a virulent strain of V. harveyi were analyzed. Luminescence and toxin production were both inhibited by the signal antagonist at concentrations that had no impact on growth. Toxin production was found to be prematurely induced in V. harveyi cultures incubated in a 10% conditioned medium. Additionally, a significant reduction in the toxicity of concentrated supernatant extracts from V. harveyi cultures incubated in the presence of the signal antagonist, as measured by in vivo toxicity assays in mice and prawns, was observed. These results suggest that intercellular signaling antagonists have potential utility in the control of V. harveyi prawn infections.

* Corresponding author. Mailing address for Michael Manefield: School of Microbiology and Immunology, University of New South Wales, Sydney 2052, Australia. Phone: 02 93852102. Fax: 02 93851591. E-mail: m.manefield{at}unsw.edu.au. Mailing address for Lachlan Harris: Department of Microbiology and Immunology, James Cook University of North Queensland, Townsville, Australia 4811. Phone: 08 40613706. Fax: 08 40613566. E-mail: lachlanharris{at}internetnorth.com.au.

Applied and Environmental Microbiology, May 2000, p. 2079-2084, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lonn-Stensrud, J., Landin, M. A., Benneche, T., Petersen, F. C., Scheie, A. A. (2009). Furanones, potential agents for preventing Staphylococcus epidermidis biofilm infections?. J Antimicrob Chemother 63: 309-316 [Abstract] [Full Text]  
  • Janssens, J. C. A., Steenackers, H., Robijns, S., Gellens, E., Levin, J., Zhao, H., Hermans, K., De Coster, D., Verhoeven, T. L., Marchal, K., Vanderleyden, J., De Vos, D. E., De Keersmaecker, S. C. J. (2008). Brominated Furanones Inhibit Biofilm Formation by Salmonella enterica Serovar Typhimurium. Appl. Environ. Microbiol. 74: 6639-6648 [Abstract] [Full Text]  
  • Sanchez-Contreras, M., Bauer, W. D, Gao, M., Robinson, J. B, Allan Downie, J (2007). Quorum-sensing regulation in rhizobia and its role in symbiotic interactions with legumes. Phil Trans R Soc B 362: 1149-1163 [Abstract] [Full Text]  
  • Defoirdt, T., Crab, R., Wood, T. K., Sorgeloos, P., Verstraete, W., Bossier, P. (2006). Quorum Sensing-Disrupting Brominated Furanones Protect the Gnotobiotic Brine Shrimp Artemia franciscana from Pathogenic Vibrio harveyi, Vibrio campbellii, and Vibrio parahaemolyticus Isolates. Appl. Environ. Microbiol. 72: 6419-6423 [Abstract] [Full Text]  
  • Koch, B., Liljefors, T., Persson, T., Nielsen, J., Kjelleberg, S., Givskov, M. (2005). The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors. Microbiology 151: 3589-3602 [Abstract] [Full Text]  
  • Rasmussen, T. B., Skindersoe, M. E., Bjarnsholt, T., Phipps, R. K., Christensen, K. B., Jensen, P. O., Andersen, J. B., Koch, B., Larsen, T. O., Hentzer, M., Eberl, L., Hoiby, N., Givskov, M. (2005). Identity and effects of quorum-sensing inhibitors produced by Penicillium species. Microbiology 151: 1325-1340 [Abstract] [Full Text]  
  • Rasmussen, T. B., Bjarnsholt, T., Skindersoe, M. E., Hentzer, M., Kristoffersen, P., Kote, M., Nielsen, J., Eberl, L., Givskov, M. (2005). Screening for Quorum-Sensing Inhibitors (QSI) by Use of a Novel Genetic System, the QSI Selector. J. Bacteriol. 187: 1799-1814 [Abstract] [Full Text]  
  • Thompson, F. L., Iida, T., Swings, J. (2004). Biodiversity of Vibrios. Microbiol. Mol. Biol. Rev. 68: 403-431 [Abstract] [Full Text]  
  • Ren, D., Bedzyk, L. A., Setlow, P., England, D. F., Kjelleberg, S., Thomas, S. M., Ye, R. W., Wood, T. K. (2004). Differential Gene Expression To Investigate the Effect of (5Z)-4-Bromo- 5-(Bromomethylene)-3-Butyl-2(5H)-Furanone on Bacillus subtilis. Appl. Environ. Microbiol. 70: 4941-4949 [Abstract] [Full Text]  
  • Wu, H., Song, Z., Hentzer, M., Andersen, J. B., Molin, S., Givskov, M., Hoiby, N. (2004). Synthetic furanones inhibit quorum-sensing and enhance bacterial clearance in Pseudomonas aeruginosa lung infection in mice. J Antimicrob Chemother 53: 1054-1061 [Abstract] [Full Text]  
  • Stroeher, U. H., Paton, A. W., Ogunniyi, A. D., Paton, J. C. (2003). Mutation of luxS of Streptococcus pneumoniae Affects Virulence in a Mouse Model. Infect. Immun. 71: 3206-3212 [Abstract] [Full Text]  
  • Long, R. A., Qureshi, A., Faulkner, D. J., Azam, F. (2003). 2-n-Pentyl-4-Quinolinol Produced by a Marine Alteromonas sp. and Its Potential Ecological and Biogeochemical Roles. Appl. Environ. Microbiol. 69: 568-576 [Abstract] [Full Text]  
  • Reimmann, C., Ginet, N., Michel, L., Keel, C., Michaux, P., Krishnapillai, V., Zala, M., Heurlier, K., Triandafillu, K., Harms, H., Defago, G., Haas, D. (2002). Genetically programmed autoinducer destruction reduces virulence gene expression and swarming motility in Pseudomonas aeruginosa PAO1. Microbiology 148: 923-932 [Abstract] [Full Text]  
  • Hentzer, M., Riedel, K., Rasmussen, T. B., Heydorn, A., Andersen, J. B., Parsek, M. R., Rice, S. A., Eberl, L., Molin, S., Hoiby, N., Kjelleberg, S., Givskov, M. (2002). Inhibition of quorum sensing in Pseudomonas aeruginosa biofilm bacteria by a halogenated furanone compound. Microbiology 148: 87-102 [Abstract] [Full Text]  
  • McDougald, D., Rice, S. A., Kjelleberg, S. (2001). SmcR-Dependent Regulation of Adaptive Phenotypes in Vibrio vulnificus. J. Bacteriol. 183: 758-762 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»