This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wiedmann, M.
Right arrow Articles by Boor, K. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wiedmann, M.
Right arrow Articles by Boor, K. J.
Agricola
Right arrow Articles by Wiedmann, M.
Right arrow Articles by Boor, K. J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, May 2000, p. 2085-2095, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular and Phenotypic Characterization of Pseudomonas spp. Isolated from Milk

Martin Wiedmann, Denise Weilmeier, Sean S. Dineen, Robert Ralyea, and Kathryn J. Boor*

Department of Food Science, Cornell University, Ithaca, New York 14853

Received 30 June 1999/Accepted 19 November 1999

Putative Pseudomonas spp. isolated predominantly from raw and processed milk were characterized by automated ribotyping and by biochemical reactions. Isolates were biochemically profiled using the Biolog system and API 20 NE and by determining the production of proteases, lipases, and lecithinases for each isolate. Isolates grouped into five coherent clusters, predominated by the species P. putida (cluster A), P. fluorescens (cluster B), P. fragi (as identified by Biolog) or P. fluorescens (as identified by API 20 NE) (cluster C), P. fragi (as identified by Biolog) or P. putida (as identified by API 20 NE) (cluster D), and P. fluorescens (cluster E). Isolates within each cluster also displayed similar enzyme activities. Isolates in clusters A, C, and D were generally negative for all three enzyme activities; isolates in cluster B were predominantly positive for all three enzyme activities; and isolates in cluster E were negative for lecithinase but predominantly positive for protease and lipase activities. Thus, only isolates from clusters B and E produced enzyme activities associated with dairy product flavor defects. Thirty-eight ribogroups were differentiated among the 70 isolates. Ribotyping was highly discriminatory for dairy Pseudomonas isolates, with a Simpson's index of discrimination of 0.955. Isolates of the same ribotype were never classified into different clusters, and ribotypes within a given cluster generally showed similar ribotype patterns; thus, specific ribotype fragments may be useful markers for tracking the sources of pseudomonads in dairy production systems. Our results suggest that ribogroups are generally homogeneous with respect to nomenspecies and biovars, confirming the identification potential of ribotyping for Pseudomonas spp.

* Corresponding author. Mailing address: Department of Food Science, 413 Stocking Hall, Cornell University, Ithaca, NY 14853. Phone: (607) 255-3111. Fax: (607) 254-4868. E-mail: kjb4{at}cornell.edu.

Applied and Environmental Microbiology, May 2000, p. 2085-2095, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Almeida, C., Azevedo, N. F., Iversen, C., Fanning, S., Keevil, C. W., Vieira, M. J. (2009). Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Cronobacter Genomospecies (Enterobacter sakazakii) in Powdered Infant Formula. Appl. Environ. Microbiol. 75: 2925-2930 [Abstract] [Full Text]  
  • Hantsis-Zacharov, E., Halpern, M. (2007). Culturable Psychrotrophic Bacterial Communities in Raw Milk and Their Proteolytic and Lipolytic Traits. Appl. Environ. Microbiol. 73: 7162-7168 [Abstract] [Full Text]  
  • Ercolini, D., Russo, F., Blaiotta, G., Pepe, O., Mauriello, G., Villani, F. (2007). Simultaneous Detection of Pseudomonas fragi, P. lundensis, and P. putida from Meat by Use of a Multiplex PCR Assay Targeting the carA Gene. Appl. Environ. Microbiol. 73: 2354-2359 [Abstract] [Full Text]  
  • Werner, B. G., Hotchkiss, J. H. (2006). Continuous flow nonthermal CO2 processing: the lethal effects of subcritical and supercritical CO2 on total microbial populations and bacterial spores in raw milk.. J DAIRY SCI 89: 872-881 [Abstract] [Full Text]  
  • Meyer, A. F., Lipson, D. A., Martin, A. P., Schadt, C. W., Schmidt, S. K. (2004). Molecular and Metabolic Characterization of Cold-Tolerant Alpine Soil Pseudomonas Sensu Stricto. Appl. Environ. Microbiol. 70: 483-489 [Abstract] [Full Text]  
  • Martin, J. D., Werner, B. G., Hotchkiss, J. H. (2003). Effects of Carbon Dioxide on Bacterial Growth Parameters in Milk as Measured by Conductivity. J DAIRY SCI 86: 1932-1940 [Abstract] [Full Text]  
  • Dogan, B., Boor, K. J. (2003). Genetic Diversity and Spoilage Potentials among Pseudomonas spp. Isolated from Fluid Milk Products and Dairy Processing Plants. Appl. Environ. Microbiol. 69: 130-138 [Abstract] [Full Text]  
  • Gunasekera, T. S., Sorensen, A., Attfield, P. V., Sorensen, S. J., Veal, D. A. (2002). Inducible Gene Expression by Nonculturable Bacteria in Milk after Pasteurization. Appl. Environ. Microbiol. 68: 1988-1993 [Abstract] [Full Text]  
  • Worm, J., Nybroe, O. (2001). Input of Protein to Lake Water Microcosms Affects Expression of Proteolytic Enzymes and the Dynamics of Pseudomonas spp.. Appl. Environ. Microbiol. 67: 4955-4962 [Abstract] [Full Text]  
  • De Cesare, A., Bruce, J. L., Dambaugh, T. R., Guerzoni, M. E., Wiedmann, M. (2001). Automated Ribotyping Using Different Enzymes To Improve Discrimination of Listeria monocytogenes Isolates, with a Particular Focus on Serotype 4b Strains. J. Clin. Microbiol. 39: 3002-3005 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»