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Applied and Environmental Microbiology, May 2000, p. 2232-2234, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Enzyme Diffusion from Trichoderma atroviride (= T. harzianum P1) to Rhizoctonia solani Is a Prerequisite for Triggering of Trichoderma ech42 Gene Expression before Mycoparasitic Contact

Cornelia Kullnig,¹ Robert L. Mach,¹ Matteo Lorito,² and Christian P. Kubicek^1,*

Section Microbial Biochemistry, Institute for Biochemical Technology and Microbiology, TU Wien, A-1060 Vienna, Austria,¹ and Dipartimento di Arboricultura, Botanica e Patologia Vegetale, Sezione di Patologia Vegetale, Università degli Studi di Napoli "Federico II," and Centro di Studio CNR per le Tecniche CNR di Lotta Biologica, 80050 Portici, Italy²

Received 23 July 1999/Accepted 15 February 2000

A plate confrontation experiment is commonly used to study the mechanism by which Trichoderma spp. antagonize and parasitize other fungi. Previous work with chitinase gene expression (ech42) during the precontact period of this process in which cellophane and dialysis membranes separated Trichoderma harzianum and its host Rhizoctonia solani resulted in essentially opposite results. Here, we show that cellophane membranes are permeable to proteins up to at least 90 kDa in size but that dialysis membranes are not. ech42 was expressed during the precontact stage of the confrontation between Trichoderma atroviride and its host only if the cellophane was placed between the two fungi. These results are consistent with enzyme diffusion from T. atroviride to R. solani generating the trigger of ech42 gene expression.

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^* Corresponding author. Mailing address: Sektion Mikrobielle Biochemie, Institut für Biochemische Technologie und Mikrobiologie, TU Wien, Getreidemarkt 9/172-5, A-1060 Vienna, Austria. Phone: 43-1 58801 17250. Fax: 43-1 581 62 66. E-mail: ckubicek{at}mail.zserv.tuwien.ac.at.

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Applied and Environmental Microbiology, May 2000, p. 2232-2234, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

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