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Applied and Environmental Microbiology, May 2000, p. 2267-2268, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of Integrated Cell Culture-PCR To Evaluate the Effectiveness of Poliovirus Inactivation by Chlorine

Felisa Blackmer, Kelly A. Reynolds,* Charles P. Gerba, and Ian L. Pepper

Department of Soil, Water and Environmental Science, The University of Arizona, Tucson, Arizona 85721

Received 5 January 1999/Accepted 1 February 2000

Current standards, based on cell culture assay, indicate that poliovirus is inactivated by 0.5 mg of free chlorine per liter after 2 min; however, integrated cell culture-PCR detected viruses for up to 8 min of exposure to the same chlorine concentration, requiring 10 min for complete inactivation. Thus, the contact time for chlorine disinfection of poliovirus is up to five times greater than previously thought.

* Corresponding author. Mailing address: Department of Soil, Water and Environmental Science, University of Arizona, 429 Shantz Building 38, Tucson, AZ 85721. Phone: (520) 419-2051. Fax: (520) 621-1647. E-mail: reynolds{at}u.arizona.edu.

Applied and Environmental Microbiology, May 2000, p. 2267-2268, Vol. 66, No. 5
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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