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Applied and Environmental Microbiology, June 2000, p. 2290-2296, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Distribution of Aldoxime Dehydratase in Microorganisms

Yasuo Kato, Ryoko Ooi, and Yasuhisa Asano*

Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Kosugi, Toyama 939-0398, Japan

Received 20 December 1999/Accepted 21 March 2000

The distribution of phenylacetaldoxime-degrading and pyridine-3-aldoxime-degrading ability was examined with intact cells of 975 microorganisms, including 45 genera of bacteria, 11 genera of actinomyces, 22 genera of yeasts, and 37 genera of fungi, by monitoring the decrease of the aldoximes by high-pressure liquid chromatography. The abilities were found to be widely distributed in bacteria, actinomyces, fungi, and some yeasts: 98 and 107 strains degraded phenylacetaldoxime and pyridine-3-aldoxime, respectively. All of the active strains exhibited not only the aldoxime-dehydration activity to form nitrile but also nitrile-hydrolyzing activity. On the other hand, all of 19 nitrile-degrading microorganisms (13 species, 7 genera) were found to exhibit aldoxime dehydration activity. It is shown that aldoxime dehydratase and nitrile-hydrolyzing activities are widely distributed among 188 aldoxime and 19 nitrile degraders and that the enzymes were induced by aldoximes or nitriles.

* Corresponding author. Mailing address: Biotechnology Research Center, Faculty of Engineering, Toyama Prefectural University, Kosugi, Toyama 939-0398, Japan. Phone: 81-766-56-7500, ext. 530. Fax: 81-766-56-2498. E-mail: asano{at}pu-toyama.ac.jp.

Applied and Environmental Microbiology, June 2000, p. 2290-2296, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Sawai, H., Sugimoto, H., Kato, Y., Asano, Y., Shiro, Y., Aono, S. (2009). X-ray Crystal Structure of Michaelis Complex of Aldoxime Dehydratase. J. Biol. Chem. 284: 32089-32096 [Abstract] [Full Text]  
  • Brandao, P. F. B., Clapp, J. P., Bull, A. T. (2003). Diversity of Nitrile Hydratase and Amidase Enzyme Genes in Rhodococcus erythropolis Recovered from Geographically Distinct Habitats. Appl. Environ. Microbiol. 69: 5754-5766 [Abstract] [Full Text]  
  • Oinuma, K.-I., Hashimoto, Y., Konishi, K., Goda, M., Noguchi, T., Higashibata, H., Kobayashi, M. (2003). Novel Aldoxime Dehydratase Involved in Carbon-Nitrogen Triple Bond Synthesis of Pseudomonas chlororaphis B23: SEQUENCING, GENE EXPRESSION, PURIFICATION, AND CHARACTERIZATION. J. Biol. Chem. 278: 29600-29608 [Abstract] [Full Text]  


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