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Applied and Environmental Microbiology, June 2000, p. 2297-2301, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Reductive Dehalogenation of Trichloroacetic Acid by Trichlorobacter thiogenes gen. nov., sp. nov.

Helene De Wever,* James R. Cole, Michael R. Fettig,dagger Deborah A. Hogan, and James M. Tiedje

Michigan State University, Center for Microbial Ecology, East Lansing, Michigan 48824-1325

Received 3 December 1999/Accepted 21 March 2000

A bacterium able to grow via reductive dechlorination of trichloroacetate was isolated from anaerobic soil enrichments. The isolate, designated strain K1, is a member of the delta  proteobacteria and is related to other known sulfur and ferric iron reducers. In anaerobic mineral media supplemented with acetate and trichloroacetate, its doubling time was 6 h. Alternative electron donor and acceptors were acetoin and sulfur or fumarate, respectively. Trichloroacetate dehalogenation activity was constitutively present, and the dechlorination product was dichloroacetate and chloride. Trichloroacetate conversion seemed to be coupled to a novel sulfur-sulfide redox cycle, which shuttled electrons from acetate oxidation to trichloroacetate reduction. In view of its unique physiological characteristics, the name Trichlorobacter thiogenes is suggested for strain K1.

* Corresponding author. Mailing address: Katholieke Universiteit Leuven, Lab for Soil Fertility and Soil Biology, Kardinaal Mercierlaan 92, 3001 Heverlee, Belgium. Phone: 32-16-32-9676. Fax: 32-16-32-1997. E-mail: heleen.dewever{at}agr.kuleuven.ac.be.

dagger Present address: ParkeDavis Co., Rochester, MI 48307.

Applied and Environmental Microbiology, June 2000, p. 2297-2301, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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