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Applied and Environmental Microbiology, June 2000, p. 2479-2483, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Hydroxylated Metabolites of 2,4-Dichlorophenol Imply a Fenton-Type Reaction in Gloeophyllum striatumdagger

Dietmar Schlosser,1,* Kristina Fahr,2 Wolfgang Karl,3 and Heinz-Georg Wetzstein4

UFZ Centre for Environmental Research Leipzig-Halle, D-06120 Halle,1 Department of Cell and Molecular Biology, Hans-Knöll-Institut für Naturstoff-Forschung e.V., D-07745 Jena,2 and Central Research3 and Animal Health Research and Development,4 Bayer AG, D-51368 Leverkusen, Germany

Received 28 December 1999/Accepted 4 April 2000

While degrading 2,4-dichlorophenol, two strains of Gloeophyllum striatum, a basidiomycetous fungus causing brown rot decay of wood, simultaneously produced 4-chlorocatechol and 3,5-dichlorocatechol. These metabolites were identified by comparing high-performance liquid chromatography retention times and mass spectral data with those of chemically synthesized standards. Under similar conditions, 3-hydroxyphthalic hydrazide was generated from phthalic hydrazide, a reaction assumed to indicate hydroxyl radical formation. Accordingly, during chemical degradation of 2,4-dichlorophenol by Fenton's reagent, identical metabolites were formed. Both activities, the conversion of 2,4-[U-14C]dichlorophenol into 14CO2 and the generation of 3-hydroxyphthalic hydrazide, were strongly inhibited by the hydroxyl radical scavenger mannitol and in the absence of iron. These results provide new evidence in favor of a Fenton-type degradation mechanism operative in Gloeophyllum.

* Corresponding author. Mailing address: UFZ Centre for Environmental Research Leipzig-Halle, Microbiology of Subterrestrial Aquatic Systems, Theodor-Lieser-Strasse 4, D-06120 Halle, Germany. Phone: 49 345 5585 204. Fax: 49 345 5585 559. E-mail: schloss{at}hdg.ufz.de.

dagger Dedicated to G. Gottschalk, University of Göttingen, on the occasion of his 65th birthday.

Applied and Environmental Microbiology, June 2000, p. 2479-2483, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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