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Applied and Environmental Microbiology, June 2000, p. 2690-2692, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Human and Animal Rotavirus Sequences in Drinking Water

B. Gratacap-Cavallier,1,* O. Genoulaz,1 K. Brengel-Pesce,1 H. Soule,1 P. Innocenti-Francillard,1 M. Bost,2 L. Gofti,3 D. Zmirou,3 and J. M. Seigneurin1

Laboratoire de Virologie1 and Service de Pédiatrie,2 Centre Hospitalier Universitaire, 38043 Grenoble, and Laboratoire de Santé Publique, Faculté de Médecine, 38000 Grenoble,3 France

Received 3 December 1999/Accepted 30 March 2000

Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplified fragments---three of them of animal origin (porcine or bovine) and one of human origin.


* Corresponding author. Mailing address: Laboratoire de Virologie, Centre Hospitalier Universitaire, 38043 Grenoble Cedex 09, France. Phone: 33 4 76 76 56 04. Fax: 33 4 76 76 52 28. E-mail: BGratacap{at}chu-grenoble.fr.


Applied and Environmental Microbiology, June 2000, p. 2690-2692, Vol. 66, No. 6
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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