This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Guan, L. L.
Right arrow Articles by Kamino, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Guan, L. L.
Right arrow Articles by Kamino, K.
Agricola
Right arrow Articles by Guan, L. L.
Right arrow Articles by Kamino, K.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2000, p. 2797-2803, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Bacterial Growth Stimulation with Exogenous Siderophore and Synthetic N-Acyl Homoserine Lactone Autoinducers under Iron-Limited and Low-Nutrient Conditions

Le Luo Guan,* Hiroyuki Onuki, and Kei Kamino

Shimizu Laboratories, Marine Biotechnology Institute, Shimizu City, Shizuoka 424-0037, Japan

Received 24 January 2000/Accepted 24 April 2000

The growth of marine bacteria under iron-limited conditions was investigated. Neither siderophore production nor bacterial growth was detected for Pelagiobacter sp. strain V0110 when Fe(III) was present in the culture medium at a concentration of <1.0 µM. However, the growth of V0110 was strongly stimulated by the presence of trace amounts of exogenous siderophore from an alpha proteobacterium, V0902, and 1 nM N-acyl-octanoylhomoserine lactone (C8-HSL), which is known as a quorum-sensing chemical signal. Even though the iron-binding functionality of a hydroxamate siderophore was undetected in the supernatant of V0902, a hydroxamate siderophore was detected in the supernatant of V0110 under the above conditions. These results indicated that hydroxamate siderophore biosynthesis by V0110 began in response to the exogenous siderophore from V0902 when in the presence of C8-HSL; however, C8-HSL production by V0110 and V0902 was not detected. Direct interaction between V0902 and V0110 through siderophore from V0902 was observed in the dialyzing culture. Similar stimulated growth by exogenous siderophore and HSL was also observed in other non-siderophore-producing bacteria isolated from marine sponges and seawater. The requirement of an exogenous siderophore and an HSL for heterologous siderophore production indicated the possibility that cell-cell communication between different species was occurring.

* Corresponding author. Shimizu Laboratories, Marine Biotechnology Institute, 1900 Sodeshi-cho, Shimizu City, Shizuoka 424-0037, Japan. Phone: 81-543-66-9215. Fax: 81-543-66-9256. E-mail: lguan{at}shimizu.mbio.co.jp.

Applied and Environmental Microbiology, July 2000, p. 2797-2803, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Nichols, D., Lewis, K., Orjala, J., Mo, S., Ortenberg, R., O'Connor, P., Zhao, C., Vouros, P., Kaeberlein, T., Epstein, S. S. (2008). Short Peptide Induces an "Uncultivable" Microorganism To Grow In Vitro. Appl. Environ. Microbiol. 74: 4889-4897 [Abstract] [Full Text]  
  • Taylor, M. W., Schupp, P. J., Baillie, H. J., Charlton, T. S., de Nys, R., Kjelleberg, S., Steinberg, P. D. (2004). Evidence for Acyl Homoserine Lactone Signal Production in Bacteria Associated with Marine Sponges. Appl. Environ. Microbiol. 70: 4387-4389 [Abstract] [Full Text]  
  • Bruns, A., Nubel, U., Cypionka, H., Overmann, J. (2003). Effect of Signal Compounds and Incubation Conditions on the Culturability of Freshwater Bacterioplankton. Appl. Environ. Microbiol. 69: 1980-1989 [Abstract] [Full Text]  
  • Fong, K. P., Gao, L., Demuth, D. R. (2003). luxS and arcB Control Aerobic Growth of Actinobacillus actinomycetemcomitans under Iron Limitation. Infect. Immun. 71: 298-308 [Abstract] [Full Text]  
  • Bruns, A., Cypionka, H., Overmann, J. (2002). Cyclic AMP and Acyl Homoserine Lactones Increase the Cultivation Efficiency of Heterotrophic Bacteria from the Central Baltic Sea. Appl. Environ. Microbiol. 68: 3978-3987 [Abstract] [Full Text]  
  • Guan, L. L., Kanoh, K., Kamino, K. (2001). Effect of Exogenous Siderophores on Iron Uptake Activity of Marine Bacteria under Iron-Limited Conditions. Appl. Environ. Microbiol. 67: 1710-1717 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»