This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heyen, U.
Right arrow Articles by Harder, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heyen, U.
Right arrow Articles by Harder, J.
Agricola
Right arrow Articles by Heyen, U.
Right arrow Articles by Harder, J.

 Previous Article  |  Next Article 

Applied and Environmental Microbiology, July 2000, p. 3004-3009, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Geranic Acid Formation, an Initial Reaction of Anaerobic Monoterpene Metabolism in Denitrifying Alcaligenes defragrans

Udo Heyen and Jens Harder*

Department of Microbiology, Max-Planck-Institute for Marine Microbiology, D-28359 Bremen, Germany

Received 14 February 2000/Accepted 8 May 2000

Monoterpenes with an unsaturated hydrocarbon structure are mineralized anaerobically by the denitrifying beta -proteobacterium Alcaligenes defragrans. Organic acids occurring in cells of A. defragrans and culture medium were characterized to identify potential products of the monoterpene activation reaction. Geranic acid (E,E-3,7-dimethyl-2,6-octadienoic acid) accumulated to 0.5 mM in cells grown on alpha -phellandrene under nitrate limitation. Cell suspensions of A. defragrans 65Phen synthesized geranic acid in the presence of beta -myrcene, alpha -phellandrene, limonene, or alpha -pinene. Myrcene yielded the highest transformation rates. The alicyclic acid was consumed by cell suspensions during carbon limitation. Heat-labile substances present in cytosolic extracts catalyzed the formation of geranic acid from myrcene. These results indicated that a novel monoterpene degradation pathway must be present in A. defragrans.

* Corresponding author. Mailing address: Department of Microbiology, Max-Planck-Institute for Marine Microbiology, Celsiusstr. 1, D-28359 Bremen, Germany. Phone: 49-421-2028-750. Fax: 49-421-2028-580. E-mail: jharder{at}mpi-bremen.de.

Applied and Environmental Microbiology, July 2000, p. 3004-3009, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Forster-Fromme, K., Chattopadhyay, A., Jendrossek, D. (2008). Biochemical characterization of AtuD from Pseudomonas aeruginosa, the first member of a new subgroup of acyl-CoA dehydrogenases with specificity for citronellyl-CoA. Microbiology 154: 789-796 [Abstract] [Full Text]  
  • Chiang, Y.-R., Ismail, W., Muller, M., Fuchs, G. (2007). Initial Steps in the Anoxic Metabolism of Cholesterol by the Denitrifying Sterolibacterium denitrificans. J. Biol. Chem. 282: 13240-13249 [Abstract] [Full Text]  
  • Diaz-Perez, A. L., Zavala-Hernandez, A. N., Cervantes, C., Campos-Garcia, J. (2004). The gnyRDBHAL Cluster Is Involved in Acyclic Isoprenoid Degradation in Pseudomonas aeruginosa. Appl. Environ. Microbiol. 70: 5102-5110 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»