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Applied and Environmental Microbiology, July 2000, p. 3073-3077, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Intracellular Bacteria in the Buds of Scotch Pine (Pinus sylvestris L.) by In Situ Hybridization

Anna Maria Pirttilä,1,2,* Hanna Laukkanen,1 Helmut Pospiech,2 Raili Myllylä,2 and Anja Hohtola1

Department of Biology/Botany1 and Department of Biochemistry,2 University of Oulu, Oulu, Finland

Received 25 October 1999/Accepted 10 April 2000

Bacterial isolates were obtained from pine (Pinus sylvestris L.) tissue cultures and identified as Methylobacterium extorquens and Pseudomonas synxantha. The existence of bacteria in pine buds was investigated by 16S rRNA in situ hybridization. Bacteria inhabited the buds of every tree examined, primarily colonizing the cells of scale primordia and resin ducts.

* Corresponding author. Mailing address: Department of Biology/Botany, P.O.B. 3000, FIN-90014 University of Oulu, Finland. Phone: 358-8-553 1547. Fax: 358-8-553 1500. E-mail: AM.Pirttila{at}oulu.fi.

Applied and Environmental Microbiology, July 2000, p. 3073-3077, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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