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Applied and Environmental Microbiology, July 2000, p. 3113-3116, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli

Anke Henne,1 Ruth A. Schmitz,1 Mechthild Bömeke,2 Gerhard Gottschalk,1,2 and Rolf Daniel1,*

Abteilung Allgemeine Mikrobiologie1 and Göttingen Genomics Laboratory,2 Institut für Mikrobiologie und Genetik der Georg-August-Universität, 37077 Göttingen, Germany

Received 24 January 2000/Accepted 5 April 2000

Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.

* Corresponding author. Mailing address: Institut für Mikrobiologie und Genetik der Georg-August-Universität, Grisebachstr. 8, 37077 Göttingen, Germany. Phone: 49-551-393827. Fax: 49-551-393808. E-mail: rdaniel{at}gwdg.de.

Applied and Environmental Microbiology, July 2000, p. 3113-3116, Vol. 66, No. 7
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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