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Applied and Environmental Microbiology, August 2000, p. 3249-3254, Vol. 66, No. 8
School of Biosciences, The University of
Birmingham, Edgbaston, Birmingham B15 2TT, United
Kingdom,1 and Department of Chemical and
Nuclear Engineering, The University of New Mexico, Albuquerque, New
Mexico 871312
Received 30 March 2000/Accepted 16 May 2000
We investigated surface selection and adhesion of motile zoospores
of a green, macrofouling alga (Enteromorpha) to
self-assembled monolayers (SAMs) having a range of wettabilities. The
SAMs were formed from alkyl thiols terminated with methyl
(CH3) or hydroxyl (OH) groups or mixtures of
CH3- and OH-terminated alkyl thiols and were characterized
by measuring the advancing contact angles and by X-ray photoelectron
spectroscopy. There was a positive correlation between the number of
spores that attached to the SAMs and increasing contact angle
(hydrophobicity). Moreover, the sizes of the spore groups (adjacent
spores touching) were larger on the hydrophobic SAMs. Video microscopy
of a patterned arrangement of SAMs showed that more zoospores were
engaged in swimming and "searching" above the hydrophobic sectors
than above the hydrophilic sectors, suggesting that the cells were able
to "sense" that the hydrophobic surfaces were more favorable for settlement. The results are discussed in relation to the attachment of
microorganisms to substrata having different wettabilities.
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Use of Self-Assembled Monolayers of Different
Wettabilities To Study Surface Selection and Primary Adhesion Processes
of Green Algal (Enteromorpha) Zoospores
*
Corresponding author. Mailing address: School of
Biosciences, The University of Birmingham, Edgbaston, Birmingham B15
2TT, United Kingdom. Phone: 44 121 414 5579. Fax: 44 121 414 5925. E-mail: m.e.callow{at}bham.ac.uk.
Applied and Environmental Microbiology, August 2000, p. 3249-3254, Vol. 66, No. 8
0099-2240/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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